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Literature summary for 1.4.1.8 extracted from

  • Vancura, A.; Vancurova, I.; Volc, J.; Fussey, S.P.M.; Flieger, M.; Neuzil, J.; Marsalek, J.; Behal, V.
    Valine dehydrogenase from Streptomyces fradiae: purification and properties (1988), J. Gen. Microbiol., 134, 3213-3219.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol presence of 2-mercaptoethanol or dithiothreitol at 10 mM required for maximal activity Streptomyces fradiae
dithiothreitol presence of 2-mercaptoethanol or dithiothreitol at 10 mM required for maximal activity Streptomyces fradiae

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ HgCl2: strong inhibition, partially abolished in presence of 2-mercaptoethanol Streptomyces fradiae
p-chloromercuribenzoate strong inhibition, partially abolished in presence of 2-mercaptoethanol Streptomyces fradiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
NAD+
-
Streptomyces fradiae
0.05
-
NADH
-
Streptomyces fradiae
0.67
-
L-leucine
-
Streptomyces fradiae
0.8
-
2-oxoisovalerate
-
Streptomyces fradiae
0.82
-
2-oxoisocaproate
-
Streptomyces fradiae
1
-
L-valine
-
Streptomyces fradiae
1
-
2-oxobutyrate
-
Streptomyces fradiae
1.18
-
L-isoleucine
-
Streptomyces fradiae
1.33
-
L-norvaline
-
Streptomyces fradiae
1.94
-
2-oxovalerate
-
Streptomyces fradiae
2.23
-
2-oxocaproate
-
Streptomyces fradiae
3.07
-
L-2-aminobutyrate
-
Streptomyces fradiae
3.33
-
2-oxo-3-methylvalerate
-
Streptomyces fradiae
6.68
-
L-norleucine
-
Streptomyces fradiae
22
-
NH4+
-
Streptomyces fradiae
25
-
pyruvate
-
Streptomyces fradiae
28.2
-
L-alanine
-
Streptomyces fradiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
12 * 18000, SDS-PAGE Streptomyces fradiae
215000
-
equilibrium ultracentrifugation Streptomyces fradiae
218000
-
gel filtration Streptomyces fradiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-valine + NAD(P)+ + H2O Streptomyces fradiae regulatory enzyme involved in biosynthesis of n-butyrate, a building unit of the oligoketide antibiotic tylosin, NAD+ as natural cofactor for oxidative deamination 2-oxoisovalerate + NH3 + NAD(P)H
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces fradiae
-
-
-
Streptomyces fradiae 30/3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
1508fold purification Streptomyces fradiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
additional information
-
-
Streptomyces fradiae

Storage Stability

Storage Stability Organism
-20┬░C, 0.2 M Tris-HCl buffer, pH 7.4, 3 months, no loss of activity Streptomyces fradiae
-20┬░C, 70% (NH4)2SO4 suspension in 0.2 M Tris-HCl buffer, 5 months, stable Streptomyces fradiae
4┬░C, 0.2 M Tris-HCl buffer, pH 7.4, 24 h, no loss of activity Streptomyces fradiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-aminobutyrate + H2O + NAD+ 68.9% of the activity with L-valine Streptomyces fradiae 2-oxobutyrate + NADH + NH3 reductive amination: 78% of the activity with 2-oxoisovalerate r
L-2-aminobutyrate + H2O + NAD+ 68.9% of the activity with L-valine Streptomyces fradiae 2-oxobutyrate + NADH + NH3 2-oxobutyrate is identical with alpha-ketobutyrate r
L-2-aminobutyrate + H2O + NAD+ L-2-aminobutyrate is identical with L-alpha-aminobutyrate Streptomyces fradiae 2-oxobutyrate + NADH + NH3 reductive amination: 78% of the activity with 2-oxoisovalerate r
L-2-aminobutyrate + H2O + NAD+ L-2-aminobutyrate is identical with L-alpha-aminobutyrate Streptomyces fradiae 2-oxobutyrate + NADH + NH3 2-oxobutyrate is identical with alpha-ketobutyrate r
L-alanine + H2O + NAD+ 14.7% of the activity with L-valine Streptomyces fradiae pyruvate + NH3 + NADH pyruvate is identical with alpha-ketopropanoate and 2-oxopropanoate r
L-alanine + H2O + NAD+ 14.7% of the activity with L-valine Streptomyces fradiae pyruvate + NH3 + NADH reductive amination: 31.6% of the activity with 2-oxoisovalerate r
L-isoleucine + H2O + NAD+ 29.4% of the activity with L-valine Streptomyces fradiae 3-methyl-2-oxopentanoate + NADH + NH3 2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate r
L-isoleucine + H2O + NAD+ 29.4% of the activity with L-valine Streptomyces fradiae 3-methyl-2-oxopentanoate + NADH + NH3 reductive amination: 23.2% of the activity with 2-oxoisovalerate r
L-leucine + H2O + NAD+ 24.5% of the activity with L-valine Streptomyces fradiae 2-oxoisocaproate + NADH + NH3 reductive amination: 20% of the activity with 2-oxoisovalerate r
L-leucine + H2O + NAD+ 24.5% of the activity with L-valine Streptomyces fradiae 2-oxoisocaproate + NADH + NH3 2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate r
L-norleucine + H2O + NAD+ 51.5% of the activity with L-valine Streptomyces fradiae 2-oxocaproate + NADH + NH3 2-oxocaproate is identical with 2-oxohexanoate and alpha-keto-n-hexanoate r
L-norleucine + H2O + NAD+ 51.5% of the activity with L-valine Streptomyces fradiae 2-oxocaproate + NADH + NH3 reductive amination: 43.8% of the activity with 2-oxoisovalerate r
L-norvaline + H2O + NAD+ 98% of the activity with L-valine Streptomyces fradiae 2-oxovalerate + NH3 + NADH reductive amination: 79.5% of the activity with 2-oxoisovalerate r
L-norvaline + H2O + NAD+ 98% of the activity with L-valine Streptomyces fradiae 2-oxovalerate + NH3 + NADH 2-oxovalerate is identical with 2-oxopentanoate and alpha-ketovalerate r
L-valine + NAD(P)+ + H2O NAD+ Streptomyces fradiae 2-oxoisovalerate + NH3 + NAD(P)H NADH r
L-valine + NAD(P)+ + H2O NAD+ Streptomyces fradiae 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
L-valine + NAD(P)+ + H2O NAD+ Streptomyces fradiae 2-oxoisovalerate + NH3 + NAD(P)H reductive amination: preferred substrate r
L-valine + NAD(P)+ + H2O NADP+ Streptomyces fradiae 2-oxoisovalerate + NH3 + NAD(P)H NADH r
L-valine + NAD(P)+ + H2O NADP+ Streptomyces fradiae 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
L-valine + NAD(P)+ + H2O NADP+ Streptomyces fradiae 2-oxoisovalerate + NH3 + NAD(P)H reductive amination: preferred substrate r
L-valine + NAD(P)+ + H2O regulatory enzyme involved in biosynthesis of n-butyrate, a building unit of the oligoketide antibiotic tylosin, NAD+ as natural cofactor for oxidative deamination Streptomyces fradiae 2-oxoisovalerate + NH3 + NAD(P)H
-
?
L-valine + NAD(P)+ + H2O NAD+ Streptomyces fradiae 30/3 2-oxoisovalerate + NH3 + NAD(P)H NADH r
L-valine + NAD(P)+ + H2O NAD+ Streptomyces fradiae 30/3 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
L-valine + NAD(P)+ + H2O NAD+ Streptomyces fradiae 30/3 2-oxoisovalerate + NH3 + NAD(P)H reductive amination: preferred substrate r
L-valine + NAD(P)+ + H2O NADP+ Streptomyces fradiae 30/3 2-oxoisovalerate + NH3 + NAD(P)H NADH r
L-valine + NAD(P)+ + H2O NADP+ Streptomyces fradiae 30/3 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r

Subunits

Subunits Comment Organism
dodecamer 12 * 18000, SDS-PAGE Streptomyces fradiae

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
assay at Streptomyces fradiae
65
-
-
Streptomyces fradiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.7 8.9 reductive amination of 2-oxoisovalerate, in presence of 0.3 M Tris-HCl buffer Streptomyces fradiae
10.5 10.7 oxidative deamination of L-valine, in presence of 0.3 M glycine-KCl-KOH buffer Streptomyces fradiae

Cofactor

Cofactor Comment Organism Structure
1,N6-etheno-NAD+ 64.4% of the activity with NAD+ Streptomyces fradiae
3-acetylpyridine-NAD+ 64.5% of the activity with NAD+ Streptomyces fradiae
Alpha-NAD+ 3.6% of the activity with NAD+ Streptomyces fradiae
deamino-NAD+ VDH2: 124% of the activity with NAD+ Streptomyces fradiae
deamino-NAD+ 93.9% of the activity with NAD+ Streptomyces fradiae
NAD+ natural cofactor for oxidative deamination Streptomyces fradiae
NADH NADH could not be replaced with NADPH for the reductive amination Streptomyces fradiae
NADP+ 10.8% of the activity with NAD+ Streptomyces fradiae
nicotinamide guanine dinucleotide 92.9% of the activity with NAD+ Streptomyces fradiae