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Literature summary for 1.4.1.21 extracted from

  • Li, H.; Zhu, T.; Miao, L.; Zhang, D.; Li, Y.; Li, Q.; Li, Y.
    Discovery of novel highly active and stable aspartate dehydrogenases (2017), Sci. Rep., 7, 7881 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Klebsiella pneumoniae
expression in Escherichia coli Delftia sp. Cs1-4

Organism

Organism UniProt Comment Textmining
Delftia sp. Cs1-4 F6ALN7
-
-
Klebsiella pneumoniae
-
-
-
Klebsiella pneumoniae 34618
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.42
-
 deamination, pH 8.2, temperature not specified in the publication Klebsiella pneumoniae
5.1
-
 deamination, pH 8.2, temperature not specified in the publication Delftia sp. Cs1-4
26.5
-
 amination, pH 8.2, temperature not specified in the publication Klebsiella pneumoniae
32.8
-
 amination, pH 8.2, temperature not specified in the publication Delftia sp. Cs1-4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH3 + NAD(P)H + H+
-
 Klebsiella pneumoniae L-glutamate + H2O + NAD(P)+
-
 ?
2-oxoglutarate + NH3 + NAD(P)H + H+
-
 Delftia sp. Cs1-4 L-glutamate + H2O + NAD(P)+
-
 ?
2-oxoglutarate + NH3 + NAD(P)H + H+
-
 Klebsiella pneumoniae 34618 L-glutamate + H2O + NAD(P)+
-
 ?
L-alanine + H2O + NAD(P)+
-
 Klebsiella pneumoniae pyruvate + NH3 + NAD(P)H + H+
-
 ?
L-alanine + H2O + NAD(P)+
-
 Delftia sp. Cs1-4 pyruvate + NH3 + NAD(P)H + H+
-
 ?
L-alanine + H2O + NAD(P)+
-
 Klebsiella pneumoniae 34618 pyruvate + NH3 + NAD(P)H + H+
-
 ?
L-aspartate + H2O + NAD(P)+
-
 Klebsiella pneumoniae oxaloacetate + NH3 + NAD(P)H + H+
-
 ?
L-aspartate + H2O + NAD(P)+
-
 Delftia sp. Cs1-4 oxaloacetate + NH3 + NAD(P)H + H+
-
 ?
L-aspartate + H2O + NAD(P)+
-
 Klebsiella pneumoniae 34618 oxaloacetate + NH3 + NAD(P)H + H+
-
 ?
L-glutamate + H2O + NAD(P)+
-
 Klebsiella pneumoniae 2-oxoglutarate + NH3 + NAD(P)H + H+
-
 ?
L-glutamate + H2O + NAD(P)+
-
 Delftia sp. Cs1-4 2-oxoglutarate + NH3 + NAD(P)H + H+
-
 ?
L-glutamate + H2O + NAD(P)+
-
 Klebsiella pneumoniae 34618 2-oxoglutarate + NH3 + NAD(P)H + H+
-
 ?
oxaloacetate + NH3 + NAD(P)H + H+
-
 Klebsiella pneumoniae L-aspartate + H2O + NAD(P)+
-
 ?
oxaloacetate + NH3 + NAD(P)H + H+
-
 Delftia sp. Cs1-4 L-aspartate + H2O + NAD(P)+
-
 ?
oxaloacetate + NH3 + NAD(P)H + H+
-
 Klebsiella pneumoniae 34618 L-aspartate + H2O + NAD(P)+
-
 ?
pyruvate + NH3 + NAD(P)H + H+
-
 Klebsiella pneumoniae L-alanine + H2O + NAD(P)+
-
 ?
pyruvate + NH3 + NAD(P)H + H+
-
 Delftia sp. Cs1-4 L-alanine + H2O + NAD(P)+
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Klebsiella pneumoniae
35
-
-
 Delftia sp. Cs1-4

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 20 min, 75% loss of activity Klebsiella pneumoniae