General Stability | Organism |
---|---|
20% glycerol, 0.25 M sodium malonate or 0.5 M sodium glutarate stabilize the enzyme during storage at 4°C | Dietzia maris |
unstable in absence of glycerol and salt | Dietzia maris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-Phenylpropionate | competitive with L-Phe | Dietzia maris | |
4-hydroxyphenylethylamine | competitive with L-Phe | Dietzia maris | |
alpha-methyl-DL-phenylalanine | - |
Dietzia maris | |
Cu2+ | slight | Dietzia maris | |
D-ethionine | - |
Dietzia maris | |
D-Phe | competitive with L-Phe | Dietzia maris | |
D-Tyr | competitive with L-Phe | Dietzia maris | |
HgCl2 | 0.01 mM, complete inhibition | Dietzia maris | |
L-Phe | product inhibition | Dietzia maris | |
L-phenylglycine | competitive with L-Phe | Dietzia maris | |
NAD+ | product inhibition | Dietzia maris | |
PCMB | 0.001 mM, complete inhibition | Dietzia maris | |
phenylenediamine | - |
Dietzia maris | |
Phenylethylamine | competitive with L-Phe | Dietzia maris | |
trans-cinnamate | competitive with L-Phe | Dietzia maris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
deamino-NAD+ | - |
Dietzia maris | |
0.18 | - |
thionicotinamide-NAD+ | - |
Dietzia maris | |
0.25 | - |
NAD+ | - |
Dietzia maris | |
0.25 | - |
3-acetylpyridine-NAD+ | - |
Dietzia maris | |
0.5 | - |
phenylpyruvate | - |
Dietzia maris | |
1.3 | - |
4-hydroxyphenylpyruvate | - |
Dietzia maris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
2 * 36000, SDS-PAGE | Dietzia maris |
70000 | - |
gel filtration | Dietzia maris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dietzia maris | - |
K-18 | - |
Dietzia maris K-18 | - |
K-18 | - |
Purification (Comment) | Organism |
---|---|
- |
Dietzia maris |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-phenylalanine + H2O + NAD+ = phenylpyruvate + NH3 + NADH + H+ | the reductive amination proceeds through a sequential ordered ternary-binary mechanism | Dietzia maris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
65.2 | - |
- |
Dietzia maris |
Storage Stability | Organism |
---|---|
4°C, 0.25 M sodium malonate, stable for 1 month | Dietzia maris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylpyruvate + NH3 + NADH + H+ | - |
Dietzia maris | L-tyrosine + NAD+ + H2O | - |
r | |
L-alpha-amino-beta-phenylbutyrate + H2O + NAD+ | 7.0% of the activity with L-Phe | Dietzia maris | 2-oxo-3-phenylbutyrate + NADH + NH3 | - |
? | |
L-ethionine + H2O + NAD+ | 13.0% of the activity with L-Phe | Dietzia maris | 4-ethylthio-2-oxobutanoate + NH3 + NADH | - |
? | |
L-norleucine + H2O + NAD+ | 15.6% of the activity with L-Phe | Dietzia maris | 2-oxohexanoic acid + NADH + NH3 | - |
? | |
L-Phe + H2O + NAD+ | the reductive amination proceeds through a sequential ordered ternary-binary mechanism | Dietzia maris | phenylpyruvate + NH3 + NADH | - |
r | |
L-Phe + H2O + NAD+ | the reductive amination proceeds through a sequential ordered ternary-binary mechanism | Dietzia maris K-18 | phenylpyruvate + NH3 + NADH | - |
r | |
L-phenylalanine + H2O + 3-acetylpyridine-NAD+ | at 241% of the activity with NAD+ | Dietzia maris | phenylpyruvate + NH3 + acetylpyridine-NADH | - |
? | |
L-phenylalanine + H2O + 3-acetylpyridine-NAD+ | at 241% of the activity with NAD+ | Dietzia maris K-18 | phenylpyruvate + NH3 + acetylpyridine-NADH | - |
? | |
L-phenylalanine + H2O + 3-pyridinealdehyde-NAD+ | at 9.2% of the activity with NAD+ | Dietzia maris | phenylpyruvate + NH3 + 3-pyridinealdehyde-NADH | - |
? | |
L-phenylalanine + H2O + deamino-NAD+ | at 86% of the activity with NAD+ | Dietzia maris | phenylpyruvate + NH3 + deamino-NADH | - |
? | |
L-phenylalanine + H2O + deamino-NAD+ | at 86% of the activity with NAD+ | Dietzia maris K-18 | phenylpyruvate + NH3 + deamino-NADH | - |
? | |
L-phenylalanine + H2O + NADP+ | at 6.6% of the activity with NAD+ | Dietzia maris | phenylpyruvate + NH3 + NADPH | - |
? | |
L-phenylalanine + H2O + NADP+ | at 6.6% of the activity with NAD+ | Dietzia maris K-18 | phenylpyruvate + NH3 + NADPH | - |
? | |
L-phenylalanine + H2O + thionicotinamide-NAD+ | at 101% of the activity with NAD+ | Dietzia maris | phenylpyruvate + NH3 + thionicotinamide-NADH | - |
? | |
L-phenylalanine + H2O + thionicotinamide-NAD+ | at 101% of the activity with NAD+ | Dietzia maris K-18 | phenylpyruvate + NH3 + thionicotinamide-NADH | - |
? | |
L-Trp + H2O + NAD+ | 7.5% of the activity with L-Phe | Dietzia maris | indole-3-pyruvate + NH3 + NADH | - |
? | |
m-fluoro-DL-phenylalanine + H2O + NAD+ | 7.7% of the activity with L-Phe | Dietzia maris | 3-(3-fluorophenyl)-2-oxopropionate + NADH + NH3 | - |
? | |
p-fluoro-DL-phenylalanine + H2O + NAD+ | 8.3% of the activity with L-Phe | Dietzia maris | (4-fluorophenyl)-2-oxopropionate + NADH + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36000, SDS-PAGE | Dietzia maris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.8 | - |
reductive amination of phenylpyruvate | Dietzia maris |
10.8 | - |
oxidative deamination of L-Phe | Dietzia maris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
3-acetylpyridine-NAD+ | 241% of the activity with NAD+ in the reaction with L-Phe | Dietzia maris | |
3-pyridinealdehyde-NAD+ | 9.2% of the activity with NAD+ in the reaction with L-Phe | Dietzia maris | |
deamino-NAD+ | 86% of the activity with NAD+ in the reaction with L-Phe | Dietzia maris | |
NAD+ | - |
Dietzia maris | |
NADH | - |
Dietzia maris | |
thionicotinamide-NAD+ | 101% of the activity with NAD+ in the reaction with L-Phe | Dietzia maris |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
D-Phe | - |
Dietzia maris | |
1.52 | - |
Phenylethylamine | - |
Dietzia maris | |
1.58 | - |
D-Tyr | - |
Dietzia maris | |
1.76 | - |
D-ethionine | - |
Dietzia maris | |
2.6 | - |
3-Phenylpropionate | - |
Dietzia maris | |
4.36 | - |
phenylenediamine | - |
Dietzia maris | |
8.32 | - |
L-phenylglycine | - |
Dietzia maris | |
12.7 | - |
trans-cinnamic acid | - |
Dietzia maris | |
13.7 | - |
4-hydroxyphenylethylamine | - |
Dietzia maris | |
20.8 | - |
alpha-methyl-DL-phenylalanine | - |
Dietzia maris |