Application | Comment | Organism |
---|---|---|
additional information | reactivation of D165N is a consequence of the catalytic chemistry of the enzymes active site | [Clostridium] symbiosum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli TG1 | [Clostridium] symbiosum |
Protein Variants | Comment | Organism |
---|---|---|
D165N/K125A | correctly folded, no significant deamidation | [Clostridium] symbiosum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | - |
[Clostridium] symbiosum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Clostridium] symbiosum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration | [Clostridium] symbiosum |
Synonyms | Comment | Organism |
---|---|---|
glutamate dehydrogenase | - |
[Clostridium] symbiosum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | protects against targeting the Cys320 in the active site by 5,5'-dithiobis(2-nitrobenzoate) | [Clostridium] symbiosum |