Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DL-methionine DL-sulfoximine | - |
Bacillus subtilis | |
fumarate | potent inhibitor of the glutamine-dependent activity | Bacillus subtilis | |
L-glutamate | noncompetitive inhibitor with respect to both 2-oxoglutarate and L-glutamine | Bacillus subtilis | |
L-malate | potent inhibitor of the glutamine-dependent activity | Bacillus subtilis | |
L-methionine DL-sulfoximine | inhibits the glutamine-dependent reaction | Bacillus subtilis | |
NAD+ | competitive inhibition with NADH, noncompetitive inhibition with 2-oxoglutarate or L-glutamine | Bacillus subtilis | |
NADP+ | competitive inhibition with NADPH, noncompetitive inhibition with 2-oxoglutarate or L-glutamine | Bacillus subtilis | |
p-chloromercuribenzoate | inhibits the glutamine-dependent reaction | Bacillus subtilis | |
pyridoxal-5'-phosphate | 52% inhibition of the glutamine-dependent reaction at 5 mM | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
NADPH | in the presence of a fixed concentration of L-glutamine, and varied concentrations of alpha-ketoglutarate | Bacillus subtilis | |
0.007 | - |
NADPH | in the presence of a fixed concentration of alpha-ketoglutarate, and varied concentrations of L-glutamine | Bacillus subtilis | |
0.007 | - |
alpha-ketoglutarate | in the presence of a fixed concentration of NADPH, and varied concentrations of L-glutamine | Bacillus subtilis | |
0.01 | - |
alpha-ketoglutarate | in the presence of a fixed concentration of NADH, and varied concentrations of L-glutamine | Bacillus subtilis | |
0.012 | - |
alpha-ketoglutarate | in the presence of a fixed concentration of L-glutamine, and varied concentrations of NADH | Bacillus subtilis | |
0.013 | - |
alpha-ketoglutarate | in the presence of a fixed concentration of L-glutamine, and varied concentrations of NADPH | Bacillus subtilis | |
0.091 | - |
NADH | in the presence of a fixed concentration of L-glutamine, and varied concentrations of alpha-ketoglutarate | Bacillus subtilis | |
0.102 | - |
L-glutamine | in the presence of a fixed concentration of NADPH, and varied concentrations of alpha-ketoglutarate | Bacillus subtilis | |
0.108 | - |
L-glutamine | in the presence of a fixed concentration of NADH, and varied concentrations of alpha-ketoglutarate | Bacillus subtilis | |
0.113 | - |
NADH | in the presence of a fixed concentration of alpha-ketoglutarate, and varied concentrations of L-glutamine | Bacillus subtilis | |
0.115 | - |
L-glutamine | in the presence of a fixed concentration of alpha-ketoglutarate, and varied concentrations of NADPH | Bacillus subtilis | |
0.12 | - |
L-glutamine | in the presence of a fixed concentration of alpha-ketoglutarate, and varied concentrations of NADH | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | iron-sulfur protein | Bacillus subtilis | |
Iron | 7.4 g-atoms of non-heme iron per mol of enzyme | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
1 * 160000 + 1 * 56000 | Bacillus subtilis |
160000 | - |
1 * 160000 + 1 * 56000 | Bacillus subtilis |
210000 | - |
sucrose density sedimentation | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+ | mechanism | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + NADH + H+ | - |
Bacillus subtilis | L-glutamate + NAD+ | - |
r | |
L-glutamine + 2-oxoglutarate + NADPH + H+ | - |
Bacillus subtilis | L-glutamate + NADP+ | - |
? | |
NH3 + 2-oxoglutarate + NADPH + H+ | 24% relative activity to L-glutamine with NADPH as electron donor and 6.3% relative activity to L-glutamine with NADH as electron donor | Bacillus subtilis | L-glutamate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 160000 + 1 * 56000 | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
NADH-glutamine dependent activity | Bacillus subtilis |
7.8 | - |
NADPH-glutamine dependent activity | Bacillus subtilis |
9.4 | - |
NADPH-NH3 dependent activity | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | 0.83 mol of FMN and 0.81 mol of FAD per mol of enzyme | Bacillus subtilis | |
additional information | the enzyme contains 8.7 g atoms per mol | Bacillus subtilis | |
NADH | 21% relative activity to NADPH | Bacillus subtilis | |
NADPH | - |
Bacillus subtilis |