BRENDA - Enzyme Database
show all sequences of 1.4.1.10

ald of Mycobacterium tuberculosis encodes both the alanine dehydrogenase and the putative glycine dehydrogenase

Giffin, M.M.; Modesti, L.; Raab, R.W.; Wayne, L.G.; Sohaskey, C.D.; J. Bacteriol. 194, 1045-1054 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene ald, quantitative real-time PCR expression analysis, expression in Escherichia coli strain ER2566
Mycobacterium tuberculosis
Engineering
Amino acid exchange
Commentary
Organism
additional information
generation of ald knockout strain RVW7 from wild-type strain H37Rv
Mycobacterium tuberculosis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.5
-
glyoxylate
pH 8.5, 22°C, recombinant enzyme
Mycobacterium tuberculosis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
minor fraction
Mycobacterium tuberculosis
5829
-
membrane
major fraction
Mycobacterium tuberculosis
16020
-
additional information
no enzyme detected in the extracellular medium
Mycobacterium tuberculosis
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mycobacterium tuberculosis
-
gene ald
-
Mycobacterium tuberculosis H37Rv
-
gene ald
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain ER2566 by nickel affinity chromatography, native enzyme by anion exchange and hydrophobic interaction chromatography, followed by ultrafiltration
Mycobacterium tuberculosis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glyoxylate + NH3 + NADH + H+
-
725267
Mycobacterium tuberculosis
glycine + H2O + NAD+
-
-
-
ir
glyoxylate + NH3 + NADH + H+
-
725267
Mycobacterium tuberculosis H37Rv
glycine + H2O + NAD+
-
-
-
ir
additional information
the putative glycine dehydrogenase of Mycobacterium tuberculosis catalyzes the reductive amination of glyoxylate to glycine but not the reverse reaction. It exhbits both pyruvate and glyoxylate aminating activitiesand also shows alanine dehydrogenase activity, EC 1.4.1.1, to a greater extent than glyoxylate aminating activity
725267
Mycobacterium tuberculosis
?
-
-
-
-
additional information
the putative glycine dehydrogenase of Mycobacterium tuberculosis catalyzes the reductive amination of glyoxylate to glycine but not the reverse reaction. It exhbits both pyruvate and glyoxylate aminating activitiesand also shows alanine dehydrogenase activity, EC 1.4.1.1, to a greater extent than glyoxylate aminating activity
725267
Mycobacterium tuberculosis H37Rv
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at room temperature
Mycobacterium tuberculosis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
assay at
Mycobacterium tuberculosis
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Mycobacterium tuberculosis
Cloned(Commentary) (protein specific)
Commentary
Organism
gene ald, quantitative real-time PCR expression analysis, expression in Escherichia coli strain ER2566
Mycobacterium tuberculosis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Mycobacterium tuberculosis
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
generation of ald knockout strain RVW7 from wild-type strain H37Rv
Mycobacterium tuberculosis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.5
-
glyoxylate
pH 8.5, 22°C, recombinant enzyme
Mycobacterium tuberculosis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
minor fraction
Mycobacterium tuberculosis
5829
-
membrane
major fraction
Mycobacterium tuberculosis
16020
-
additional information
no enzyme detected in the extracellular medium
Mycobacterium tuberculosis
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain ER2566 by nickel affinity chromatography, native enzyme by anion exchange and hydrophobic interaction chromatography, followed by ultrafiltration
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glyoxylate + NH3 + NADH + H+
-
725267
Mycobacterium tuberculosis
glycine + H2O + NAD+
-
-
-
ir
glyoxylate + NH3 + NADH + H+
-
725267
Mycobacterium tuberculosis H37Rv
glycine + H2O + NAD+
-
-
-
ir
additional information
the putative glycine dehydrogenase of Mycobacterium tuberculosis catalyzes the reductive amination of glyoxylate to glycine but not the reverse reaction. It exhbits both pyruvate and glyoxylate aminating activitiesand also shows alanine dehydrogenase activity, EC 1.4.1.1, to a greater extent than glyoxylate aminating activity
725267
Mycobacterium tuberculosis
?
-
-
-
-
additional information
the putative glycine dehydrogenase of Mycobacterium tuberculosis catalyzes the reductive amination of glyoxylate to glycine but not the reverse reaction. It exhbits both pyruvate and glyoxylate aminating activitiesand also shows alanine dehydrogenase activity, EC 1.4.1.1, to a greater extent than glyoxylate aminating activity
725267
Mycobacterium tuberculosis H37Rv
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at room temperature
Mycobacterium tuberculosis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
assay at
Mycobacterium tuberculosis
Expression
Organism
Commentary
Expression
Mycobacterium tuberculosis
transcription of ald is induced when alanine is the sole nitrogen source
up
General Information
General Information
Commentary
Organism
physiological function
the enzyme plays an essential role in the utilization of alanine, but not of glycine, as a nitrogen source
Mycobacterium tuberculosis
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme plays an essential role in the utilization of alanine, but not of glycine, as a nitrogen source
Mycobacterium tuberculosis
Expression (protein specific)
Organism
Commentary
Expression
Mycobacterium tuberculosis
transcription of ald is induced when alanine is the sole nitrogen source
up
Other publictions for EC 1.4.1.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725267
Giffin
ald of Mycobacterium tuberculo ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Bacteriol.
194
1045-1054
2012
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-
1
-
1
-
-
1
3
-
-
-
-
162
-
-
1
-
-
-
-
-
4
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
1
3
-
-
-
-
-
-
1
-
-
-
-
4
-
1
-
-
-
1
-
-
-
1
1
1
1
-
-
724782
Ji
Glycine and a glycine dehydrog ...
Homo sapiens
Clin. Pharmacol. Ther.
89
97-104
2011
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
349594
Usha
Glycine and alanine dehydrogen ...
Mycolicibacterium smegmatis
Can. J. Microbiol.
48
7-13
2002
-
-
-
-
-
-
1
-
-
-
-
-
-
4
-
-
1
-
-
-
1
1
1
-
1
-
-
-
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
1
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
391430
Wayne
Glyoxylate metabolism and adap ...
Mycobacterium tuberculosis
Infect. Immun.
37
1042-1049
1982
-
-
-
-
-
-
1
4
-
-
-
-
-
3
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391429
Loginova
-
Enzymes of the assimilation of ...
Blastobacter aminovorus, Hyphomicrobium vulgare, no activity in Arthrobacter globiformis, no activity in Methylomonas methylovora, no activity in Methylomonas methylovora M8-5 M12-4, no activity in Methylophilus methanolovorus, Pseudomonas methylica, Pseudomonas sp., Pseudomonas sp. AM1, Xanthobacter viscosus
Mikrobiologiya
51
38-42
1981
-
-
-
-
-
-
-
-
-
-
-
-
-
17
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
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5
-
-
-
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-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
391428
Goldman
Enzyme systems in the Mycobact ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Ra / ATCC 25177
Biochim. Biophys. Acta
65
297-306
1962
-
-
-
-
-
-
2
2
-
-
-
2
-
10
-
-
1
-
-
-
1
1
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
2
-
-
-
1
-
-
1
1
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-