Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-cyano-3-hydroxy-N-(2',3,3'-trichlorobiphenyl-4-yl)but-2-enamide | - |
Toxoplasma gondii | |
2-hydroxyethylidene-cyano acetic acid 4-trifluoromethyl anilide | A177-1726, active derivative of human inhibitor leflunomide | Toxoplasma gondii | |
additional information | not inhibitory: brequinar, atovaquone, N-(3,5-difluoro-4-(trifluoromethyl)phenyl)-5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-amine | Toxoplasma gondii | |
N-(3-chloro-2'-methoxybiphenyl-4-yl)-2-cyano-3-hydroxybut-2-enamide | - |
Toxoplasma gondii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
ubiquinone-50 | pH 8.0, 30°C | Toxoplasma gondii | |
0.028 | - |
1,4-Naphthoquinone | pH 8.0, 30°C | Toxoplasma gondii | |
0.029 | - |
decylubiquinone | pH 8.0, 30°C | Toxoplasma gondii | |
0.051 | - |
ubiquinone-30 | pH 8.0, 30°C | Toxoplasma gondii | |
0.06 | - |
(S)-dihydroorotate | pH 8.0, 30°C | Toxoplasma gondii | |
0.099 | - |
2,3-dimethoxy-5-methyl-1,4-benzoquinone | pH 8.0, 30°C | Toxoplasma gondii | |
0.132 | - |
2,5-dimethyl-p-benzoquinone | pH 8.0, 30°C | Toxoplasma gondii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Toxoplasma gondii | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
84 | - |
pH 8.0, 30°C | Toxoplasma gondii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + 1,4-naphthoquinone | - |
Toxoplasma gondii | orotate + 1,4-naphthoquinol | - |
? | |
(S)-dihydroorotate + 2,3-dimethoxy-5-methyl-1,4-benzoquinone | - |
Toxoplasma gondii | orotate + 2,3-dimethoxy-5-methyl-1,4-benzoquinol | - |
? | |
(S)-dihydroorotate + 2,5-dimethyl-p-benzoquinone | - |
Toxoplasma gondii | orotate + 2,5-dimethyl-p-benzoquinol | - |
? | |
(S)-dihydroorotate + decylubiquinone | - |
Toxoplasma gondii | orotate + decylubiquinol | - |
? | |
(S)-dihydroorotate + ubiquinone-30 | - |
Toxoplasma gondii | orotate + ubiquinol-30 | - |
? | |
(S)-dihydroorotate + ubiquinone-50 | - |
Toxoplasma gondii | orotate + ubiquinol-50 | - |
? | |
additional information | fumarate is a poor electron acceptor | Toxoplasma gondii | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21 | - |
ubiquinone-30 | pH 8.0, 30°C | Toxoplasma gondii | |
29 | - |
1,4-Naphthoquinone | pH 8.0, 30°C | Toxoplasma gondii | |
32 | - |
2,3-dimethoxy-5-methyl-1,4-benzoquinone | pH 8.0, 30°C | Toxoplasma gondii | |
44 | - |
2,5-dimethyl-p-benzoquinone | pH 8.0, 30°C | Toxoplasma gondii | |
61 | - |
ubiquinone-50 | pH 8.0, 30°C | Toxoplasma gondii | |
82 | - |
(S)-dihydroorotate | pH 8.0, 30°C | Toxoplasma gondii | |
89 | - |
decylubiquinone | pH 8.0, 30°C | Toxoplasma gondii |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.06 | - |
pH 8.0, 30°C | Toxoplasma gondii | N-(3-chloro-2'-methoxybiphenyl-4-yl)-2-cyano-3-hydroxybut-2-enamide | |
0.091 | - |
pH 8.0, 30°C | Toxoplasma gondii | 2-hydroxyethylidene-cyano acetic acid 4-trifluoromethyl anilide | |
0.096 | - |
pH 8.0, 30°C | Toxoplasma gondii | 2-cyano-3-hydroxy-N-(2',3,3'-trichlorobiphenyl-4-yl)but-2-enamide |
General Information | Comment | Organism |
---|---|---|
physiological function | the gene appears to be essential, because while gene tagging is successful at the TgDHOD gene locus, attempts to delete the TgDHOD gene were not successful in the KU80 background | Toxoplasma gondii |