Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
crystal structure of YwfH is determined in three conformational states. These represent apo YwfH, the YwfH/NADPH complex and an apo-like form | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
K113A | kcat/Km is 4.9fold lower than the wild-type value | Bacillus subtilis |
N158A | kcat/Km is 4.6fold lower than the wild-type value | Bacillus subtilis |
S155A | kcat/Km is 5.5fold lower than the wild-type value | Bacillus subtilis |
S250A | kcat/Km is 2.2fold lower than the wild-type value | Bacillus subtilis |
Y117A | kcat/Km is 5.8fold lower than the wild-type value | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.178 | - |
NADPH | pH and temperature not specified in the publication, wild-type enzyme | Bacillus subtilis | |
0.365 | - |
NADPH | pH and temperature not specified in the publication, mutant enzyme K113A | Bacillus subtilis | |
323 | - |
NADPH | pH and temperature not specified in the publication, mutant enzyme S250A | Bacillus subtilis | |
345 | - |
NADPH | pH and temperature not specified in the publication, mutant enzyme N158A | Bacillus subtilis | |
528 | - |
NADPH | pH and temperature not specified in the publication, mutant enzyme Y117A | Bacillus subtilis | |
614 | - |
NADPH | pH and temperature not specified in the publication, mutant enzyme S155A | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
2 * 31000 | Bacillus subtilis |
61500 | - |
analytical size-exclusion chromatography | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3E)-3-[(4S)-4-hydroxycyclohex-2-en-1-yl]-2-oxopropanoate + NADPH + H+ | - |
Bacillus subtilis | 3-[(1R,4S)-4-hydroxycyclohex-2-en-1-yl]-2-oxopropanoate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 31000 | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
BacG | - |
Bacillus subtilis |
YwfH | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0344 | - |
NADPH | pH and temperature not specified in the publication, wild-type enzyme | Bacillus subtilis | |
0.143 | - |
NADPH | pH and temperature not specified in the publication, mutant enzyme N158A | Bacillus subtilis | |
0.146 | - |
NADPH | pH and temperature not specified in the publication, mutant enzyme K113A | Bacillus subtilis | |
0.175 | - |
NADPH | pH and temperature not specified in the publication, mutant enzyme Y117A | Bacillus subtilis | |
0.216 | - |
NADPH | pH and temperature not specified in the publication, mutant enzyme S155A | Bacillus subtilis | |
0.286 | - |
NADPH | pH and temperature not specified in the publication, mutant enzyme S250A | Bacillus subtilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
NADPH | pH and temperature not specified in the publication, mutant enzyme Y117A | Bacillus subtilis | |
0.035 | - |
NADPH | pH and temperature not specified in the publication, mutant enzyme S155A | Bacillus subtilis | |
0.04 | - |
NADPH | pH and temperature not specified in the publication, mutant enzyme K113A | Bacillus subtilis | |
0.042 | - |
NADPH | pH and temperature not specified in the publication, mutant enzyme N158A | Bacillus subtilis | |
0.089 | - |
NADPH | pH and temperature not specified in the publication, mutant enzyme S250A | Bacillus subtilis | |
0.193 | - |
NADPH | pH and temperature not specified in the publication, wild-type enzyme | Bacillus subtilis |