Literature summary for 1.3.1.97 extracted from

Jiang, Z.; Kempinski, C.; Bush, C.J.; Nybo, S.E.; Chappell, J.
Engineering triterpene and methylated triterpene production in plants provides biochemical and physiological insights into terpene metabolism (2016), Plant Physiol., 170, 702-716 .

Application

Application	Comment	Organism
synthesis	an expression system for production of high levels of botryococcene in Nicotiana tabacum plants can be used involving the recombinant chimeric enzyme, trichome-specific expression of botryococcene metabolism, overview	Botryococcus braunii

Cloned(Commentary)

Cloned (Comment)	Organism
gene SSL-3, recombinant expression of the enzyme in Nicotiana tabacum, ene constructs consisted of a peptide fusion of SSL-1 and SSL-3 connected by a triplet repeat peptide linker of Gly-Gly-Ser-Gly, with or without appending the C-terminal end (71 amino acids) of the Botryococcus braunii squalene synthase onto the C-terminus of SSL-3 and the FPS gene. The chimeric SSL1-3 genes and FPS gene are inserted downstream of the strong constitutive promoters Pcv and Pca, respectively. For trichome-specific expression of triterpene biosynthesis, the trichome-specific promoters Pcbt and Pcyp16 are fused to 5' end of botryococcene synthase gene, presqualene diphosphate synthase gene, and the FPS gene, respectively	Botryococcus braunii

Cloned (Comment)

Organism

gene SSL-3, recombinant expression of the enzyme in Nicotiana tabacum, ene constructs consisted of a peptide fusion of SSL-1 and SSL-3 connected by a triplet repeat peptide linker of Gly-Gly-Ser-Gly, with or without appending the C-terminal end (71 amino acids) of the Botryococcus braunii squalene synthase onto the C-terminus of SSL-3 and the FPS gene. The chimeric SSL1-3 genes and FPS gene are inserted downstream of the strong constitutive promoters Pcv and Pca, respectively. For trichome-specific expression of triterpene biosynthesis, the trichome-specific promoters Pcbt and Pcyp16 are fused to 5' end of botryococcene synthase gene, presqualene diphosphate synthase gene, and the FPS gene, respectively

Botryococcus braunii

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of an expression system for production of high levels of botryococcene in Nicotiana tabacum strain KY 1068 plants by diverting carbon flux from the cytosolic mevalonate pathway or the plastidic methylerythritol phosphate pathway by the targeted overexpression of an avian farnesyl diphosphate synthase along with botryococcene synthase SSL-3 and presqualene diphosphate synthase SSL-1, overview. The structure of purified botryococcene from tobacco is determined by 1H-NMR and 13C-NMR spectral analyses. Modifications of botryococcene in tobacco by coexpressed triterpene methyltransferases (TMTs) from Botryococcus braunii	Botryococcus braunii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
presqualene diphosphate + NADPH + H+	Botryococcus braunii	-	C30 botryococcene + NADP+ + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Botryococcus braunii	G0Y288	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
presqualene diphosphate + NADPH + H+	-	Botryococcus braunii	C30 botryococcene + NADP+ + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
SSL-3	-	Botryococcus braunii

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Botryococcus braunii