Application | Comment | Organism |
---|---|---|
diagnostics | the enzyme defined as an independent prognostic factor for the development of non-small cell lung cancer cells | Homo sapiens |
medicine | the enzyme may serve as a valuable target for therapeutic intervention in pulmonary carcinogenesis | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
recombinant expression of wild-type and selenomethionine-labeled hDus2 1-340 fragment, comprising the hDus2 catalytic and tRNA-recognition domains, in Escherichia coli strains BL21(DE3) and B834(DE3), respectively | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant hDus2 catalytic and tRNA-recognition domains (residues 1-340), sitting drop vapour diffusion method, 300 nl of 10 mg/ml protein in 20 mM Tris, pH 8.0, 100 mM NaCl, 5 mM imidazole, and 5 mM DTT, are mixed with 0. 54 ml of reservoir solution containing 0.1 M MES-malic acid-Tris, pH 4.0, and 25% w/v PEG 1500, 2 days, 19°C, X-ray diffraction structure determination and analysis by single-wavelength anomalous diffraction at 1.9 A resolution, automated molecular replacement with different search models, and modeling | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | siRNA-dependent knockdown of hDus2 decreases colony formation and cell viability of non-small cell lung cancer cells, while hDus2 immunohistochemical staining correlated with patient survival | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil20 in tRNA + NAD(P)H + H+ | Homo sapiens | - |
5,6-dihydrouracil20 in tRNA + NAD(P)+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NX74 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and selenomethionine-labeled hDus2 1-340 fragment, comprising the hDus2 catalytic and tRNA-recognition domains, from Escherichia coli strains BL21(DE3) and B834(DE3), respectively | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
non-small cell lung cancer cell | elevated hDus2 mRNA and protein levels | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil20 in tRNA + NAD(P)H + H+ | - |
Homo sapiens | 5,6-dihydrouracil20 in tRNA + NAD(P)+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | three-dimensional structure analysis of wild-type and selenomethionine-labeled hDus2 catalytic and tRNA-recognition domains, and modeling, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
dihydrouridine synthase | - |
Homo sapiens |
dihydrouridine synthase 2 | - |
Homo sapiens |
Dus | - |
Homo sapiens |
hDUS2 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | In humans, there are four Dus enzymes. The hDus2 subfamily is proposed to specifically modify U20. The overall fold of the human Dus2 is similar to that of bacterial enzymes, but has a larger recognition domain and a unique three-stranded antiparallel beta-sheet insertion into the catalytic domain that packs next to the recognition domain, contributing to domain-domain interactions | Homo sapiens |
malfunction | increased expression of human dihydrouridine synthase 2 (hDus2) is linked to pulmonary carcinogenesis, while its knockdown decreases cancer cell line viability | Homo sapiens |
additional information | tRNA binding and the active site structure, overview | Homo sapiens |
physiological function | dihydrouridine is produced by dihydrouridine synthase (Dus) by enzymatic reduction of the C5-C6 bond in uridine | Homo sapiens |