Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Vitis bellula |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sodium chloride | 500 mm, almost complete loss of activity | Vitis bellula |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.131 | - |
delphinidin | pH 4.0, 40°C | Vitis bellula | |
0.135 | - |
pelargonidin | pH 4.0, 40°C | Vitis bellula | |
0.176 | - |
cyanidin | pH 4.0, 40°C | Vitis bellula | |
0.193 | - |
NADPH | pH 4.0, 40°C | Vitis bellula | |
0.817 | - |
NADH | pH 4.0, 40°C | Vitis bellula |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Vitis bellula | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
x * 56000, SDS-PAGE | Vitis bellula |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vitis bellula | H9TZS7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyanidin + 2 NADPH + 2 H+ | - |
Vitis bellula | (-)-catechin + (-)-epicatechin + 2 NADP+ | - |
? | |
delphinidin + 2 NADH + 2 H+ | - |
Vitis bellula | (-)-epigallocatechin + (-)-gallocatechin + 2 NAD+ | - |
? | |
additional information | ANR converts cyanidin, delphinidin, and pelargonidin to their corresponding flavan-3-ols. Enzymatic products include 2S,3R-trans, 2R,3R-cis and probably 2S,3S-cis-flavan-3-ol isomers | Vitis bellula | ? | - |
? | |
pelargonidin + 2 NADPH + 2 H+ | - |
Vitis bellula | (-)-epiafzelechin + (-)-afzelechin + 2 NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, SDS-PAGE | Vitis bellula |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Vitis bellula |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.66 | - |
cyanidin | pH 4.0, 40°C | Vitis bellula | |
2 | - |
pelargonidin | pH 4.0, 40°C | Vitis bellula | |
2.01 | - |
delphinidin | pH 4.0, 40°C | Vitis bellula |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Vitis bellula |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | NADPH is preferred over NADH | Vitis bellula | |
NADPH | NADPH is preferred over NADH | Vitis bellula |
General Information | Comment | Organism |
---|---|---|
physiological function | overexpression of ANR in banyuls mutants reconstructs the biosynthetic pathway of proanthocyanidins in the seed coat | Vitis bellula |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.48 | - |
cyanidin | pH 4.0, 40°C | Vitis bellula | |
14.9 | - |
pelargonidin | pH 4.0, 40°C | Vitis bellula | |
16.4 | - |
delphinidin | pH 4.0, 40°C | Vitis bellula |