BRENDA - Enzyme Database
show all sequences of 1.3.1.70

Inhibition of sterol DELTA8 -> DELTA7-isomerase and DELTA14-reductase by fenpropimorph, tridemorph and fenpropidin in cell-free enzyme systems from Saccharomyces cerevisiae

Baloch, R.I.; Mercer, E.I.; Phytochemistry 26, 663-668 (1987)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
fenpropidin
fungicide, very potent inhibitor; IC50: 0.0018 mM; N-[3-(p-tert-butylphenyl)-2-methylpropyl]-piperidine
Saccharomyces cerevisiae
fenpropidin
fungicide, very potent inhibitor; N-[3-(p-tert-butylphenyl)-2-methylpropyl]-piperidine
Ustilago maydis
fenpropimorph
fungicide, very potent inhibitor; IC50: 0.0023 mM; N-[3-(p-tert-butylphenyl)-2-methylpropyl]-cis-2,6-dimethylmorpholine
Saccharomyces cerevisiae
fenpropimorph
fungicide, very potent inhibitor; N-[3-(p-tert-butylphenyl)-2-methylpropyl]-cis-2,6-dimethylmorpholine
Ustilago maydis
tridemorph
2,6-dimethyl-N-tridecylmorpholine; fungicide; slight inhibitor, IC50: 0.098 mM
Saccharomyces cerevisiae
tridemorph
2,6-dimethyl-N-tridecylmorpholine; fungicide
Ustilago maydis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Saccharomyces cerevisiae
essential enzyme of ergosterol, yeast sterol, biosynthesis from lanosterol
?
-
-
-
additional information
Saccharomyces cerevisiae NCYC 739
essential enzyme of ergosterol, yeast sterol, biosynthesis from lanosterol
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
NCYC 739
-
Saccharomyces cerevisiae NCYC 739
-
NCYC 739
-
Ustilago maydis
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Saccharomyces cerevisiae
Reaction
Reaction
Commentary
Organism
4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP+ = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH + H+
mechanism
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5alpha-ergosta-8,14,24(28)-trien-3beta-ol + NADPH
-
288495
Saccharomyces cerevisiae
5alpha-ergosta-8,24(28)-dien-3beta-ol + NADP+
-
-
-
?
5alpha-ergosta-8,14,24(28)-trien-3beta-ol + NADPH
-
288495
Saccharomyces cerevisiae NCYC 739
5alpha-ergosta-8,24(28)-dien-3beta-ol + NADP+
-
-
-
?
additional information
essential enzyme of ergosterol, yeast sterol, biosynthesis from lanosterol
288495
Saccharomyces cerevisiae
?
-
-
-
-
additional information
essential enzyme of ergosterol, yeast sterol, biosynthesis from lanosterol
288495
Saccharomyces cerevisiae NCYC 739
?
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2
-
assay at
Saccharomyces cerevisiae
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Saccharomyces cerevisiae
NADPH
-
Ustilago maydis
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.0018
-
IC50: 0.0018 mM
Saccharomyces cerevisiae
fenpropidin
0.0023
-
IC50: 0.0023 mM
Saccharomyces cerevisiae
fenpropimorph
0.098
-
slight inhibitor, IC50: 0.098 mM
Saccharomyces cerevisiae
tridemorph
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Saccharomyces cerevisiae
NADPH
-
Ustilago maydis
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.0018
-
IC50: 0.0018 mM
Saccharomyces cerevisiae
fenpropidin
0.0023
-
IC50: 0.0023 mM
Saccharomyces cerevisiae
fenpropimorph
0.098
-
slight inhibitor, IC50: 0.098 mM
Saccharomyces cerevisiae
tridemorph
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
fenpropidin
fungicide, very potent inhibitor; IC50: 0.0018 mM; N-[3-(p-tert-butylphenyl)-2-methylpropyl]-piperidine
Saccharomyces cerevisiae
fenpropidin
fungicide, very potent inhibitor; N-[3-(p-tert-butylphenyl)-2-methylpropyl]-piperidine
Ustilago maydis
fenpropimorph
fungicide, very potent inhibitor; IC50: 0.0023 mM; N-[3-(p-tert-butylphenyl)-2-methylpropyl]-cis-2,6-dimethylmorpholine
Saccharomyces cerevisiae
fenpropimorph
fungicide, very potent inhibitor; N-[3-(p-tert-butylphenyl)-2-methylpropyl]-cis-2,6-dimethylmorpholine
Ustilago maydis
tridemorph
2,6-dimethyl-N-tridecylmorpholine; fungicide; slight inhibitor, IC50: 0.098 mM
Saccharomyces cerevisiae
tridemorph
2,6-dimethyl-N-tridecylmorpholine; fungicide
Ustilago maydis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Saccharomyces cerevisiae
essential enzyme of ergosterol, yeast sterol, biosynthesis from lanosterol
?
-
-
-
additional information
Saccharomyces cerevisiae NCYC 739
essential enzyme of ergosterol, yeast sterol, biosynthesis from lanosterol
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5alpha-ergosta-8,14,24(28)-trien-3beta-ol + NADPH
-
288495
Saccharomyces cerevisiae
5alpha-ergosta-8,24(28)-dien-3beta-ol + NADP+
-
-
-
?
5alpha-ergosta-8,14,24(28)-trien-3beta-ol + NADPH
-
288495
Saccharomyces cerevisiae NCYC 739
5alpha-ergosta-8,24(28)-dien-3beta-ol + NADP+
-
-
-
?
additional information
essential enzyme of ergosterol, yeast sterol, biosynthesis from lanosterol
288495
Saccharomyces cerevisiae
?
-
-
-
-
additional information
essential enzyme of ergosterol, yeast sterol, biosynthesis from lanosterol
288495
Saccharomyces cerevisiae NCYC 739
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2
-
assay at
Saccharomyces cerevisiae
Other publictions for EC 1.3.1.70
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746161
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744648
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Impaired cell proliferation i ...
Mus musculus
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15
2164-2173
2016
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1
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2
2
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745882
Li
Structure of an integral memb ...
Methylomicrobium alcaliphilum 20Z, Methylomicrobium alcaliphilum
Nature
517
104-107
2015
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1
1
11
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2
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6
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1
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8
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11
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8
1
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3
3
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725903
Liu
A sterol C-14 reductase encode ...
Fusarium graminearum
Microbiology
157
1665-1675
2011
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3
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2
2
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-
711302
Schiavoni
Activation of TM7SF2 promoter ...
Homo sapiens
Biochim. Biophys. Acta
1801
587-592
2010
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1
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2
1
1
2
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713446
Wang
Gonadotropin-regulated express ...
Oryctolagus cuniculus
Prostaglandins Other Lipid Mediat.
92
25-32
2010
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1
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725863
Hata
Inhibition of ergosterol synth ...
Candida albicans
Med. Mycol.
48
613-621
2010
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1
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2
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723885
Liang
2-Amino-nonyl-6-methoxyl-tetra ...
Candida albicans
Acta Pharmacol. Sin.
30
1709
2009
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1
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4
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688770
Shah Alam Bhuiyan
Synthetically lethal interacti ...
Saccharomyces cerevisiae
Lipids
42
69-76
2007
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672378
Bennati
Sterol dependent regulation of ...
Homo sapiens
Biochim. Biophys. Acta
1761
677-685
2006
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1
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22
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22
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4
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655352
Yamashita
Gonadotropin-induced DELTA14-r ...
Sus scrofa
Endocrinology
146
186-194
2005
1
-
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1
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2
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654166
Waterham
Autosomal recessive HEM/Greenb ...
Homo sapiens
Am. J. Hum. Genet.
72
1013-1017
2003
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657046
He
Sterols regulate development a ...
Arabidopsis thaliana
Plant Physiol.
131
1258-1269
2003
1
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1
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1
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3
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288492
Roberti
Cloning and expression of ster ...
Bos taurus, Homo sapiens
Eur. J. Biochem.
269
283-290
2002
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2
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6
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2
4
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7
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3
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12
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12
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654204
Jia
Candida albicans sterol C-14 r ...
Candida albicans
Antimicrob. Agents Chemother.
46
947-957
2002
-
1
1
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1
-
13
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1
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1
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656304
Prakash
Genes encoding chimeras of Neu ...
Homo sapiens, Neurospora crassa
J. Biosci.
27
105-112
2002
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2
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656798
Prakash
The sterol C-14 reductase enco ...
Neurospora crassa
Mol. Genet. Genomics
266
787-795
2002
-
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1
-
38
-
1
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1
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38
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288491
Kim
Cholesterol biosynthesis from ...
Rattus norvegicus
Biochim. Biophys. Acta
1259
39-48
1995
4
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2
7
1
2
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3
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1
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1
1
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4
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7
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1
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1
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288494
Lorenz
Cloning, sequencing, and disru ...
Saccharomyces cerevisiae
DNA Cell Biol.
11
685-692
1992
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1
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2
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2
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2
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3
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12
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4
1
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11
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