Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21(DE3) | Treponema denticola |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
crotonoyl-CoA | - |
Treponema denticola | |
additional information | shows no inhibition by triclosan in a wide range of concentration (0.005-1 mM), or FAD (0.01 mM) | Treponema denticola |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
crotonoyl-CoA | in potassium phosphate buffer pH 6.2, at 30°C | Treponema denticola |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
SDS-PAGE | Treponema denticola |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Treponema denticola | - |
strain ATCC 35405 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Treponema denticola |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
43 | - |
using crotonoyl-CoA and NADH as substrates, at 30°C | Treponema denticola |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonyl-CoA + NADH + H+ | - |
Treponema denticola | butyryl-CoA + NAD+ | - |
? | |
additional information | not active with NADPH or hexenoyl-CoA | Treponema denticola | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 44000, SDS-PAGE | Treponema denticola |
Synonyms | Comment | Organism |
---|---|---|
TER | - |
Treponema denticola |
trans-2-enoyl-CoA reductase, NADH-dependent | - |
Treponema denticola |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | dependent | Treponema denticola |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000198 | - |
crotonoyl-CoA | in potassium phosphate buffer pH 6.2, at 30°C | Treponema denticola |