General Stability | Organism |
---|---|
loss of activity in solutions with protein concentration below 0.030-0.035 mg/ml | Rattus norvegicus |
NADPH stabilizes | Rattus norvegicus |
repeated freeze-thawing: little loss of activity , no significant loss of activity, partially purified enzyme | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | pretreatment of the enzyme with NADPH and biliverdins fully protects | Rattus norvegicus | |
bilirubin | inhibits the enzyme in a competitive manner | Rattus norvegicus | |
Biliverdin | the enzyme is inhibited by the substrate when the concentration exceeds 0.004-0.005 mM | Rattus norvegicus | |
Hematin | - |
Rattus norvegicus | |
HgCl2 | irreversible inhibition | Rattus norvegicus | |
iodoacetamide | pretreatment of the enzyme with NADPH and biliverdins fully protects | Rattus norvegicus | |
Metalloporphyrin complexes | - |
Rattus norvegicus | |
N-ethylmaleimide | pretreatment of the enzyme with NADPH and biliverdins fully protects | Rattus norvegicus | |
p-chloromercuribenzoate | pretreatment of the enzyme with NADPH and biliverdins fully protects | Rattus norvegicus | |
SH-reagents | e.g. 5,5'-dithiobis(2-nitrobenzoic acid), N-ethylmaleimide, pretreatment of the enzyme with NADPH and biliverdins fully protects | Rattus norvegicus | |
zinc-protoporphyrin | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
NADPH | - |
Rattus norvegicus | |
0.003 | - |
Biliverdin | cosubstrate NADPH | Rattus norvegicus | |
0.005 | - |
Biliverdin | cosubstrate NADH | Rattus norvegicus | |
0.27 | - |
NADH | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Rattus norvegicus | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | 36000 | liver | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.5 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, several months -25°C, 1 month: no loss of activity, 2 months: 50% loss of activity | Rattus norvegicus |
0-4°C, 5 days | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bilirubin + NAD+ | - |
Rattus norvegicus | biliverdin + NADH + H+ | - |
? | |
biliverdin + NAD(P)H | - |
Rattus norvegicus | bilirubin + NAD(P)+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
10 min, no loss of activity | Rattus norvegicus |
55 | - |
10 min, complete loss of activity | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
biliverdin + NADH | Rattus norvegicus |
8.7 | - |
biliverdin + NADPH | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
0.1 M potassium phosphate buffer, most stable | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |