Cloned (Comment) | Organism |
---|---|
overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), expression of selenomethionine enzyme in Escherichia coli strain B834(DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
wild-type and selenomethionine enzyme, 0.001 ml of protein and of reservoir solution, the latter containing 16% PEG 4000 w/v, 0.18 M ammonium sulfate, 80 mM sodium acetate, pH 4.6, 20% glycerol, addition of 30% ethylene glycol and 4 mM NADP+ for the binary complex of enzyme with NADP+, and 120 mM substrate for the ternary complex of enzyme with NADP+ and substrate trans-trans-2,4-dienoyl-CoA, X-ray diffraction structure determination and analysis at 2.1 A and 1.75 A resolution, respectively | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E310A | site-directed mutagenesis, effects on reaction mechanism | Homo sapiens |
E310Q | site-directed mutagenesis, effects on reaction mechanism | Homo sapiens |
K214A | site-directed mutagenesis, effects on reaction mechanism | Homo sapiens |
N148A | site-directed mutagenesis, effects on reaction mechanism | Homo sapiens |
S210A | site-directed mutagenesis, effects on reaction mechanism | Homo sapiens |
T197A | site-directed mutagenesis, effects on reaction mechanism | Homo sapiens |
Y199A | site-directed mutagenesis, effects on reaction mechanism | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | enzyme is the key enzyme of beta-oxidation of unsaturated fatty acid in mitochondria and to a lesser extent in peroxisomes, three accessory proteins are involved in the pathway | ? | - |
? | |
trans-trans-2,4-hexadienoyl-CoA + NADPH | Homo sapiens | - |
trans-3-hexenoyl-CoA + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, to homogeneity | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a (2E)-2-enoyl-CoA + NADP+ = a (2E,4E)-2,4-dienoyl-CoA + NADPH + H+ | reaction mechanism, enzyme-ligand interactions in a distinctive short-chain reductase active site, catalytic site structure, stabilization of the dienolate reaction intermediate by Tyr199 and Asn148, and NADP+, overview | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme is the key enzyme of beta-oxidation of unsaturated fatty acid in mitochondria and to a lesser extent in peroxisomes, three accessory proteins are involved in the pathway | Homo sapiens | ? | - |
? | |
trans-trans-2,4-hexadienoyl-CoA + NADPH | - |
Homo sapiens | trans-3-hexenoyl-CoA + NADP+ | - |
r | |
trans-trans-2,4-hexadienoyl-CoA + NADPH | substrate binding mechanism and structure | Homo sapiens | trans-3-hexenoyl-CoA + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | crystal structure | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
2,4-dienoyl-CoA reductase | - |
Homo sapiens |
DECR | - |
Homo sapiens |
More | enzyme belongs to the short-chain dehydrogenase/reductase family with a distinctive catalytic center | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | binding mechanism and structure | Homo sapiens | |
NADPH | binding mechanism and structure | Homo sapiens |