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Literature summary for 1.3.1.114 extracted from

  • Harris, S.; Devendran, S.; Alves, J.; Mythen, S.; Hylemon, P.; Ridlon, J.
    Identification of a gene encoding a flavoprotein involved in bile acid metabolism by the human gut bacterium Clostridium scindens ATCC 35704 (2018), Biochim. Biophys. Acta, 1863, 276-283 .
    View publication on PubMed
Search for more literature for 1.3.1.114

Cloned(Commentary)

Cloned (Comment) Organism
expressionj in Escherichia coli [Clostridium] scindens
overexpression in Escherichia coli [Clostridium] scindens

General Stability

General Stability Organism
purified protein is stable only for less than 12 h, then precipitates [Clostridium] scindens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NAD+ [Clostridium] scindens
-
 12alpha-hydroxy-3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
 ?
12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NAD+ [Clostridium] scindens the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration 12alpha-hydroxy-3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
 ?
12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NAD+ [Clostridium] scindens ATCC 35704
-
 12alpha-hydroxy-3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
 ?
12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NAD+ [Clostridium] scindens ATCC 35704 the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration 12alpha-hydroxy-3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
 ?
12alpha-hydroxy-3-oxocholan-24-oyl-CoA + NAD+ [Clostridium] scindens
-
 12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
12alpha-hydroxy-3-oxocholan-24-oyl-CoA + NAD+ [Clostridium] scindens the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration 12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
12alpha-hydroxy-3-oxocholan-24-oyl-CoA + NAD+ [Clostridium] scindens ATCC 35704
-
 12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
12alpha-hydroxy-3-oxocholan-24-oyl-CoA + NAD+ [Clostridium] scindens ATCC 35704 the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration 12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
3-oxochol-4-en-24-oyl-CoA + NAD+ [Clostridium] scindens
-
 3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
 ?
3-oxochol-4-en-24-oyl-CoA + NAD+ [Clostridium] scindens the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration 3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
 ?
3-oxochol-4-en-24-oyl-CoA + NAD+ [Clostridium] scindens ATCC 35704
-
 3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
 ?
3-oxocholan-24-oyl-CoA + NAD+ [Clostridium] scindens
-
 3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
3-oxocholan-24-oyl-CoA + NAD+ [Clostridium] scindens the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration 3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
[Clostridium] scindens B0NAQ4
-
-
[Clostridium] scindens ATCC 35704 B0NAQ4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 [Clostridium] scindens

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol does not improve stability [Clostridium] scindens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NAD+
-
 [Clostridium] scindens 12alpha-hydroxy-3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
 ?
12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration [Clostridium] scindens 12alpha-hydroxy-3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
 ?
12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NAD+
-
 [Clostridium] scindens ATCC 35704 12alpha-hydroxy-3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
 ?
12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration [Clostridium] scindens ATCC 35704 12alpha-hydroxy-3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
 ?
12alpha-hydroxy-3-oxocholan-24-oyl-CoA + NAD+
-
 [Clostridium] scindens 12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
12alpha-hydroxy-3-oxocholan-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration [Clostridium] scindens 12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
12alpha-hydroxy-3-oxocholan-24-oyl-CoA + NAD+
-
 [Clostridium] scindens ATCC 35704 12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
12alpha-hydroxy-3-oxocholan-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration [Clostridium] scindens ATCC 35704 12alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
3-oxochol-4-en-24-oyl-CoA + NAD+
-
 [Clostridium] scindens 3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
 ?
3-oxochol-4-en-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration [Clostridium] scindens 3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
 ?
3-oxochol-4-en-24-oyl-CoA + NAD+
-
 [Clostridium] scindens ATCC 35704 3-oxochol-4,6-dien-24-oyl-CoA + NADH + H+
-
 ?
3-oxocholan-24-oyl-CoA + NAD+
-
 [Clostridium] scindens 3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?
3-oxocholan-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration [Clostridium] scindens 3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 44500, calculated from sequence, x * 46500, SDS-PAGE, recombinant protein with streptavidin-tag [Clostridium] scindens

Synonyms

Synonyms Comment Organism
BaiN
-
 [Clostridium] scindens
CLOSCI_00523
-
 [Clostridium] scindens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at [Clostridium] scindens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at [Clostridium] scindens

Cofactor

Cofactor Comment Organism Structure
FAD
-
 [Clostridium] scindens
flavin flavoenzyme [Clostridium] scindens

General Information

General Information Comment Organism
metabolism the enzyme participates in the bile acid 7alpha-dehydroxylation pathway. It catalyses two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration [Clostridium] scindens
physiological function enzyme participates in the bile acid 7alpha-dehydroxylation pathway, catalyzing two subsequent reductions of the double bonds within the bile acid A/B rings, following 7alpha-dehydration [Clostridium] scindens