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Literature summary for 1.3.1.10 extracted from
- Resistance to AFN-1252 arises from missense mutations in Staphylococcus aureus enoyl-acyl carrier protein reductase (FabI) (2013), J. Biol. Chem., 288, 36261-36271.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli JP1111 cells | Staphylococcus aureus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| M99T | the mutant is resistant toward inhibition by AFN-1252 | Staphylococcus aureus |
| Y147F | the mutant is catalytically defective but retains its sensitivity to inhibitor AFN-1252 | Staphylococcus aureus |
| Y147H | the mutant is catalytically defective but retains its sensitivity to inhibitor AFN-1252 | Staphylococcus aureus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AFN-1252 | - |
Staphylococcus aureus |  |
| triclosan | - |
Staphylococcus aureus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | - |
- |
- |
| Staphylococcus aureus RN4220 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and Superdex 200 gel filtration | Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| crotonyl-CoA + NADPH + H+ | - |
Staphylococcus aureus | ? | - |
? | |
| crotonyl-CoA + NADPH + H+ | - |
Staphylococcus aureus RN4220 | ? | - |
? | |
| crotonyl-[acyl-carrier protein] + NADPH + H+ | - |
Staphylococcus aureus | ? | - |
? | |
| crotonyl-[acyl-carrier protein] + NADPH + H+ | - |
Staphylococcus aureus RN4220 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| enoyl-[acyl-carrier-protein] reductase | - |
Staphylococcus aureus |
| FabI | - |
Staphylococcus aureus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.038 | - |
crotonyl-[acyl-carrier protein] | mutant enzyme Y147H, pH and temperature not specified in the publication | Staphylococcus aureus | |
| 0.038 | - |
crotonyl-[acyl-carrier protein] | mutant enzyme Y147F, pH and temperature not specified in the publication | Staphylococcus aureus | |
| 0.51 | - |
crotonyl-[acyl-carrier protein] | wild type enzyme, pH and temperature not specified in the publication | Staphylococcus aureus | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Staphylococcus aureus | |
| NADPH | - |
Staphylococcus aureus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000004 | - |
AFN-1252 | wild type enzyme, pH and temperature not specified in the publication | Staphylococcus aureus | |
| 0.000069 | - |
AFN-1252 | mutant enzyme M99T, pH and temperature not specified in the publication | Staphylococcus aureus | |
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