Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | optimal activity at 0.1 mM total salts | Sphingobium chlorophenolicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
non-denaturing PAGE | Sphingobium chlorophenolicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3,5,6-tetrachlorohydroquinone + 2 glutathione | Sphingobium chlorophenolicum | the enzyme is involved in degradation of pentachlorophenol | 2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide | - |
? | |
2,3,6-trichlorohydroquinone + 2 glutathione | Sphingobium chlorophenolicum | the enzyme is involved in degradation of pentachlorophenol | 2,6-dichlorohydroquinone + Cl- + glutathione disulfide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium chlorophenolicum | Q03520 | - |
- |
Oxidation Stability | Organism |
---|---|
sensitive to oxygen, a loss of enzyme activity occurrs when DTT is not included in elution buffers during purification | Sphingobium chlorophenolicum |
Purification (Comment) | Organism |
---|---|
- |
Sphingobium chlorophenolicum |
Storage Stability | Organism |
---|---|
-20°C, no measurable loss of activity over a period of 1 month | Sphingobium chlorophenolicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3,5,6-tetrachlorohydroquinone + 2 glutathione | the enzyme is involved in degradation of pentachlorophenol | Sphingobium chlorophenolicum | 2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide | - |
? | |
2,3,5,6-tetrachlorohydroquinone + 2 glutathione | the enzyme converts tetrachlorohydroquinone to trichlorohydroquinone and trichlorohydroquinone to dichlorohydroquinone. The enzyme uses glutathione but not NADPH, NADH, dithiothreitol, or ascorbic acid as the reducing agent | Sphingobium chlorophenolicum | 2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide | - |
? | |
2,3,6-trichlorohydroquinone + 2 glutathione | the enzyme is involved in degradation of pentachlorophenol | Sphingobium chlorophenolicum | 2,6-dichlorohydroquinone + Cl- + glutathione disulfide | - |
? | |
2,3,6-trichlorohydroquinone + 2 glutathione | the enzyme converts tetrachlorohydroquinone to trichlorohydroquinone and trichlorohydroquinone to dichlorohydroquinone. The enzyme uses glutathione but not NADPH, NADH, dithiothreitol, or ascorbic acid as the reducing agent | Sphingobium chlorophenolicum | 2,6-dichlorohydroquinone + Cl- + glutathione disulfide | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 30000, SDS-PAGE | Sphingobium chlorophenolicum |
Synonyms | Comment | Organism |
---|---|---|
PCP hydroxylase | - |
Sphingobium chlorophenolicum |
tetrachloro-p-hydroquinone reductive dehalogenase | - |
Sphingobium chlorophenolicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Sphingobium chlorophenolicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | - |
Sphingobium chlorophenolicum |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in degradation of pentachlorophenol | Sphingobium chlorophenolicum |