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Literature summary for 1.20.9.1 extracted from

  • Ellis, P.J.; Conrads, T.; Hille, R.; Kuhn, P.
    Crystal structure of the 100 kDa arsenite oxidase from Alcaligenes faecalis in two crystal forms at 1.64 A and 2.03 A (2001), Structure, 9, 125-132.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
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Alcaligenes faecalis
crystal structure of arsenite oxidase to 2.03A in a P21 crystal form with two molecules in the asymmetric unit and to 1.64 A in a P1 crystal form with four molecules in the asymmetric unit. The large subunit contains a Mo site, consisting of a Mo atom bound to two pterin cofactors, and a [3Fe-4S] cluster. The small subunit contains a Rieske-type [2Fe-2S] site Alcaligenes faecalis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe contains a [3Fe-4S] cluster and a Rieske-type [2Fe-2S] site Alcaligenes faecalis
Iron the large subunit contains a [3Fe-4S] cluster. The small subunit contains a Rieske-type [2Fe-2S] site Alcaligenes faecalis
Molybdenum the large subunit contains a Mo site, consisting of a Mo atom bound to two pterin cofactors Alcaligenes faecalis

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
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Alcaligenes faecalis Q7SIF3 small subunit aoxA
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Alcaligenes faecalis Q7SIF4 large subunit aoxB
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Alcaligenes faecalis NCIB 8687 Q7SIF3 small subunit aoxA
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Alcaligenes faecalis NCIB 8687 Q7SIF4 large subunit aoxB
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arsenite + H2O + azurin(oxidized)
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Alcaligenes faecalis arsenate + azurin(reduced)
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ir

Subunits

Subunits Comment Organism
More heterodimer Alcaligenes faecalis

Synonyms

Synonyms Comment Organism
aoxA
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Alcaligenes faecalis
aoxB
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Alcaligenes faecalis

Cofactor

Cofactor Comment Organism Structure
iron-sulfur centre the large subunit contains a [3Fe-4S] cluster. The small subunit contains a Rieske-type [2Fe-2S] site Alcaligenes faecalis
molybdopterin the large subunit contains a Mo site, consisting of a Mo atom bound to two pterin cofactors Alcaligenes faecalis