Cloned (Comment) | Organism |
---|---|
expression by functional complementation of the Escherichia coli gap mutant W3CG | Streptococcus pyogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | 1 mM, 85% inhibition | Streptococcus pyogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.385 | - |
NADP+ | pH 8.5, 25°C | Streptococcus pyogenes | |
0.666 | - |
D-Glyceraldehyde-3-phosphate | pH 8.5, 25°C | Streptococcus pyogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
4 * 55000, SDS-PAGE | Streptococcus pyogenes |
224000 | - |
non-denaturing PAGE | Streptococcus pyogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Streptococcus pyogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
143.2 | - |
- |
Streptococcus pyogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde-3-phosphate + NADP+ | - |
Streptococcus pyogenes | D-3-phosphoglycerate + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 55000, SDS-PAGE | Streptococcus pyogenes |
Synonyms | Comment | Organism |
---|---|---|
non-phosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase | - |
Streptococcus pyogenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Streptococcus pyogenes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
10 min, inactivation above | Streptococcus pyogenes |
75 | - |
10 min, complete inactivation | Streptococcus pyogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Streptococcus pyogenes |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 12 | pH 7.5: about 35% of maximal activity, pH 12.0: about 60% of maximal activity | Streptococcus pyogenes |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptococcus pyogenes | chromatofocusing | - |
4.9 |