Cloned (Comment) | Organism |
---|---|
- |
Cupriavidus necator |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition: acetaldehyde, phosphonoacetaldehyde, glycolaldehyde, formaldehyde, propionaldehyde, succinic semialdehyde, glyoxylate, betaine aldehyde, glyceraldehyde, and 2-oxobutanoic acid | Cupriavidus necator |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.064 | - |
NADP+ | pH 9.0, temperature not specified in the publication | Cupriavidus necator | |
0.102 | - |
CoA | pH 9.0, temperature not specified in the publication | Cupriavidus necator | |
0.33 | - |
2-sulfoacetaldehyde | pH 9.0, temperature not specified in the publication | Cupriavidus necator |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
2 * 52000, SDS-PAGE | Cupriavidus necator |
115000 | - |
gel filtration | Cupriavidus necator |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfoacetyl-CoA + NADPH + H+ | Cupriavidus necator | the enzyme is involved in degradation of sulfoacetate | 2-sulfoacetaldehyde + CoA + NADP+ | - |
? | |
sulfoacetyl-CoA + NADPH + H+ | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | the enzyme is involved in degradation of sulfoacetate | 2-sulfoacetaldehyde + CoA + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | Q0K845 | - |
- |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | Q0K845 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Cupriavidus necator |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:2-sulfoacetate-grown cell | inducible activity. Extracts of acetate-, taurine-, or isethionate-grown cells show no activity, extracts of sulfoacetate-grown cells catalyze the reaction | Cupriavidus necator | - |
Storage Stability | Organism |
---|---|
4°C or -18°C, 1 week, 30% loss of activity | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-sulfoacetaldehyde + CoA + NADP+ | specific for NADP+, no activity with NAD+. The enzyme is specific for sulfoacetaldehyde. No activity with: acetaldehyde, phosphonoacetaldehyde, glycolaldehyde, formaldehyde, propionaldehyde, succinic semialdehyde, glyoxylate, betaine aldehyde, glyceraldehyde, and 2-oxobutanoic acid | Cupriavidus necator | sulfoacetyl-CoA + NADPH + H+ | - |
r | |
2-sulfoacetaldehyde + CoA + NADP+ | specific for NADP+, no activity with NAD+. The enzyme is specific for sulfoacetaldehyde. No activity with: acetaldehyde, phosphonoacetaldehyde, glycolaldehyde, formaldehyde, propionaldehyde, succinic semialdehyde, glyoxylate, betaine aldehyde, glyceraldehyde, and 2-oxobutanoic acid | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | sulfoacetyl-CoA + NADPH + H+ | - |
r | |
sulfoacetyl-CoA + NADPH + H+ | the enzyme is involved in degradation of sulfoacetate | Cupriavidus necator | 2-sulfoacetaldehyde + CoA + NADP+ | - |
? | |
sulfoacetyl-CoA + NADPH + H+ | the enzyme is involved in degradation of sulfoacetate | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | 2-sulfoacetaldehyde + CoA + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 52000, SDS-PAGE | Cupriavidus necator |
Synonyms | Comment | Organism |
---|---|---|
SauS | - |
Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Cupriavidus necator |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | specific for NADP+, no activity with NAD+ | Cupriavidus necator |
Organism | Comment | Expression |
---|---|---|
Cupriavidus necator | inducible activity. Extracts of acetate-, taurine-, or isethionate-grown cells show no activity, extracts of sulfoacetate-grown cells catalyze the reaction | up |
General Information | Comment | Organism |
---|---|---|
malfunction | deletion mutant does not grow on sulfoacetate | Cupriavidus necator |