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Literature summary for 1.2.1.81 extracted from

  • Weinitschke, S.; Hollemeyer, K.; Kusian, B.; Bowien, B.; Smits, T.H.; Cook, A.M.
    Sulfoacetate is degraded via a novel pathway involving sulfoacetyl-CoA and sulfoacetaldehyde in Cupriavidus necator H16 (2010), J. Biol. Chem., 285, 35249-35254.
    View publication on PubMed
Search for more literature for 1.2.1.81

Cloned(Commentary)

Cloned (Comment) Organism
-
 Cupriavidus necator

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition: acetaldehyde, phosphonoacetaldehyde, glycolaldehyde, formaldehyde, propionaldehyde, succinic semialdehyde, glyoxylate, betaine aldehyde, glyceraldehyde, and 2-oxobutanoic acid Cupriavidus necator

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.064
-
 NADP+ pH 9.0, temperature not specified in the publication Cupriavidus necator
0.102
-
 CoA pH 9.0, temperature not specified in the publication Cupriavidus necator
0.33
-
 2-sulfoacetaldehyde pH 9.0, temperature not specified in the publication Cupriavidus necator

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 2 * 52000, SDS-PAGE Cupriavidus necator
115000
-
 gel filtration Cupriavidus necator

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sulfoacetyl-CoA + NADPH + H+ Cupriavidus necator the enzyme is involved in degradation of sulfoacetate 2-sulfoacetaldehyde + CoA + NADP+
-
 ?
sulfoacetyl-CoA + NADPH + H+ Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 the enzyme is involved in degradation of sulfoacetate 2-sulfoacetaldehyde + CoA + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Cupriavidus necator Q0K845
-
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 Q0K845
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cupriavidus necator

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:2-sulfoacetate-grown cell inducible activity. Extracts of acetate-, taurine-, or isethionate-grown cells show no activity, extracts of sulfoacetate-grown cells catalyze the reaction Cupriavidus necator
-

Storage Stability

Storage Stability Organism
4°C or -18°C, 1 week, 30% loss of activity Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-sulfoacetaldehyde + CoA + NADP+ specific for NADP+, no activity with NAD+. The enzyme is specific for sulfoacetaldehyde. No activity with: acetaldehyde, phosphonoacetaldehyde, glycolaldehyde, formaldehyde, propionaldehyde, succinic semialdehyde, glyoxylate, betaine aldehyde, glyceraldehyde, and 2-oxobutanoic acid Cupriavidus necator sulfoacetyl-CoA + NADPH + H+
-
 r
2-sulfoacetaldehyde + CoA + NADP+ specific for NADP+, no activity with NAD+. The enzyme is specific for sulfoacetaldehyde. No activity with: acetaldehyde, phosphonoacetaldehyde, glycolaldehyde, formaldehyde, propionaldehyde, succinic semialdehyde, glyoxylate, betaine aldehyde, glyceraldehyde, and 2-oxobutanoic acid Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 sulfoacetyl-CoA + NADPH + H+
-
 r
sulfoacetyl-CoA + NADPH + H+ the enzyme is involved in degradation of sulfoacetate Cupriavidus necator 2-sulfoacetaldehyde + CoA + NADP+
-
 ?
sulfoacetyl-CoA + NADPH + H+ the enzyme is involved in degradation of sulfoacetate Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 2-sulfoacetaldehyde + CoA + NADP+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 52000, SDS-PAGE Cupriavidus necator

Synonyms

Synonyms Comment Organism
SauS
-
 Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Cupriavidus necator

Cofactor

Cofactor Comment Organism Structure
NADP+ specific for NADP+, no activity with NAD+ Cupriavidus necator

Expression

Organism Comment Expression
Cupriavidus necator inducible activity. Extracts of acetate-, taurine-, or isethionate-grown cells show no activity, extracts of sulfoacetate-grown cells catalyze the reaction up

General Information

General Information Comment Organism
malfunction deletion mutant does not grow on sulfoacetate Cupriavidus necator