Cloned (Comment) | Organism |
---|---|
gene HEMA1, recombinant co-overexpression of N-terminally His6-tagged FLUTPR and GluTRDD in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
uncomplexed TPR domain of FLU (FLUTPR) and complex of the dimeric domain of GluTR bound to FLUTPR, hanging drop vapor diffusion method, from 0.2 M NaCl, 0.1 M Bis-Tris, pH 6.5, and 25% w/v PEG 3350 in 1 week, and from 0.15 M KBr and 30% w/v PEG monomethyl ether 2000, in 3 weeks, X-ray diffraction structure determination and analysis at 1.45 and 2.4 A resolution, respectively, molecular replacement and modeling | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
protein FLU | the non-canonical tetratricopeptide repeat (TPR) domain of fluorescent (FLU) mediates complex formation with glutamyl-tRNA reductase. Protein FLU negatively regulates glutamyl-tRNA reductase (GluTR) during chlorophyll biosynthesis. A 2:2 FLUTPR-GluTR complex is the functional unit for FLU-mediated GluTR regulation. Enzyme binding complex structure analysis from crystal structures, detailed overview | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu | Arabidopsis thaliana | - |
L-glutamyl-tRNAGlu + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P42804 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged FLUTPR and GluTRDD in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu | - |
Arabidopsis thaliana | L-glutamyl-tRNAGlu + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GluTR | - |
Arabidopsis thaliana |
HEMA1 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
physiological function | protein FLU negatively regulates glutamyl-tRNA reductase (GluTR) during chlorophyll biosynthesis. It directly interacts through its TPR domain with glutamyl-tRNA reductase (GluTR), the rate-limiting enzyme in the formation of 5-aminolevulinic acid. The formation of the FLU-GluTR complex prevents glutamyl-tRNA, the GluTR substrate, from binding with this enzyme | Arabidopsis thaliana |