Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 100% inhibition with 1 mM | Burkholderia cepacia | |
Ag+ | 100% inhibition with 1 mM | Micrococcus sp. | |
EDTA | 40% inhibition by 1 mM and 45% inhibition at 5 mM | Micrococcus sp. | |
Mg2+ | 1.9fold increased enzyme activity with 1 mM | Micrococcus sp. | |
additional information | no effect of EDTA on enzyme activity; no inhibition with 1 mM Cu2+, Fe3+, Fe2+, Zn2+, Mn2+, and Co2+ | Burkholderia cepacia | |
additional information | no inhibition with 1 mM Cu2+, Fe3+, Fe2+, Zn2+, Mn2+, and Co2+ | Micrococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
Vanillin | pH 7, temperature not specified in the publication | Burkholderia cepacia | |
0.007 | - |
Vanillin | pH 7, temperature not specified in the publication | Micrococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
2 * 57000, SDS-PAGE | Burkholderia cepacia |
57000 | - |
4 * 57000, SDS-PAGE | Micrococcus sp. |
110000 | - |
gel filtration | Burkholderia cepacia |
250000 | - |
gel filtration | Micrococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | - |
- |
- |
Burkholderia cepacia TM1 | - |
- |
- |
Micrococcus sp. | - |
- |
- |
Micrococcus sp. TA1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by DEAE-Sepharose FF column, butyl-Sepharose column and Resource Q column chromatography | Burkholderia cepacia |
by DEAE-Sepharose FF column, butyl-Sepharose column and Resource Q column chromatography | Micrococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzaldehyde + NADP+ + H2O | 70% relative activity compared to vanillin | Burkholderia cepacia | 4-hydroxybenzoate + NADPH + 2 H+ | - |
? | |
4-hydroxybenzaldehyde + NADP+ + H2O | 77% relative activity compared to vanillin | Micrococcus sp. | 4-hydroxybenzoate + NADPH + 2 H+ | - |
? | |
4-hydroxybenzaldehyde + NADP+ + H2O | 77% relative activity compared to vanillin | Micrococcus sp. TA1 | 4-hydroxybenzoate + NADPH + 2 H+ | - |
? | |
4-hydroxybenzaldehyde + NADP+ + H2O | 70% relative activity compared to vanillin | Burkholderia cepacia TM1 | 4-hydroxybenzoate + NADPH + 2 H+ | - |
? | |
isovanillin + NADP+ + H2O | 285% relative activity compared to vanillin | Micrococcus sp. | isovanillate + NADPH + 2 H+ | - |
? | |
isovanillin + NADP+ + H2O | 48% relative activity compared to vanillin | Burkholderia cepacia | isovanillate + NADPH + 2 H+ | - |
? | |
isovanillin + NADP+ + H2O | 285% relative activity compared to vanillin | Micrococcus sp. TA1 | isovanillate + NADPH + 2 H+ | - |
? | |
isovanillin + NADP+ + H2O | 48% relative activity compared to vanillin | Burkholderia cepacia TM1 | isovanillate + NADPH + 2 H+ | - |
? | |
protocatechualdehyde + NADP+ + H2O | 146% relative activity compared to vanillin | Micrococcus sp. | protocatechuate + NADPH + 2 H+ | - |
? | |
protocatechualdehyde + NADP+ + H2O | 94% relative activity compared to vanillin | Burkholderia cepacia | protocatechuate + NADPH + 2 H+ | - |
? | |
protocatechualdehyde + NADP+ + H2O | 146% relative activity compared to vanillin | Micrococcus sp. TA1 | protocatechuate + NADPH + 2 H+ | - |
? | |
protocatechualdehyde + NADP+ + H2O | 94% relative activity compared to vanillin | Burkholderia cepacia TM1 | protocatechuate + NADPH + 2 H+ | - |
? | |
syringaldehyde + NADP+ + H2O | 23% relative activity compared to vanillin | Micrococcus sp. | syringate + NADPH + 2 H+ | - |
? | |
syringaldehyde + NADP+ + H2O | 29% relative activity compared to vanillin | Burkholderia cepacia | syringate + NADPH + 2 H+ | - |
? | |
syringaldehyde + NADP+ + H2O | 23% relative activity compared to vanillin | Micrococcus sp. TA1 | syringate + NADPH + 2 H+ | - |
? | |
syringaldehyde + NADP+ + H2O | 29% relative activity compared to vanillin | Burkholderia cepacia TM1 | syringate + NADPH + 2 H+ | - |
? | |
vanillin + NAD+ + H2O | higher activity with NAD+ than with NADP+, relative activity with NADP1 is approximately 10% | Burkholderia cepacia | vanillate + NADH + 2 H+ | - |
? | |
vanillin + NAD+ + H2O | higher activity with NAD+ than with NADP+, relative activity with NADP1 is approximately 10% | Burkholderia cepacia TM1 | vanillate + NADH + 2 H+ | - |
? | |
vanillin + NADP+ + H2O | no activity with NAD+, strain TA1 exhibits oxidation activity specifically under alkaline conditions | Micrococcus sp. | vanillate + NADPH + 2 H+ | - |
? | |
vanillin + NADP+ + H2O | no activity with NAD+, strain TA1 exhibits oxidation activity specifically under alkaline conditions | Micrococcus sp. TA1 | vanillate + NADPH + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 57000, SDS-PAGE | Burkholderia cepacia |
homotetramer | 4 * 57000, SDS-PAGE | Micrococcus sp. |
Synonyms | Comment | Organism |
---|---|---|
VDH | - |
Burkholderia cepacia |
VDH | - |
Micrococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Micrococcus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 60 | high activity across a wide range of temperature | Burkholderia cepacia |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
stable up to 30 min | Micrococcus sp. |
35 | - |
stable up to 30 min | Burkholderia cepacia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 10 | - |
Burkholderia cepacia |
9 | 10 | - |
Micrococcus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | 30-min incubation at 30°C | Burkholderia cepacia |
7 | 8 | 30 min incubation at 30°C | Micrococcus sp. |