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Literature summary for 1.2.1.67 extracted from

  • Mitsui, R.; Hirota, M.; Tsuno, T.; Tanaka, M.
    Purification and characterization of vanillin dehydrogenases from alkaliphile Micrococcus sp. TA1 and neutrophile Burkholderia cepacia TM1 (2010), FEMS Microbiol. Lett., 303, 41-47.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 100% inhibition with 1 mM Burkholderia cepacia
Ag+ 100% inhibition with 1 mM Micrococcus sp.
EDTA 40% inhibition by 1 mM and 45% inhibition at 5 mM Micrococcus sp.
Mg2+ 1.9fold increased enzyme activity with 1 mM Micrococcus sp.
additional information no effect of EDTA on enzyme activity; no inhibition with 1 mM Cu2+, Fe3+, Fe2+, Zn2+, Mn2+, and Co2+ Burkholderia cepacia
additional information no inhibition with 1 mM Cu2+, Fe3+, Fe2+, Zn2+, Mn2+, and Co2+ Micrococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
Vanillin pH 7, temperature not specified in the publication Burkholderia cepacia
0.007
-
Vanillin pH 7, temperature not specified in the publication Micrococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
2 * 57000, SDS-PAGE Burkholderia cepacia
57000
-
4 * 57000, SDS-PAGE Micrococcus sp.
110000
-
gel filtration Burkholderia cepacia
250000
-
gel filtration Micrococcus sp.

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
-
-
Burkholderia cepacia TM1
-
-
-
Micrococcus sp.
-
-
-
Micrococcus sp. TA1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by DEAE-Sepharose FF column, butyl-Sepharose column and Resource Q column chromatography Burkholderia cepacia
by DEAE-Sepharose FF column, butyl-Sepharose column and Resource Q column chromatography Micrococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxybenzaldehyde + NADP+ + H2O 70% relative activity compared to vanillin Burkholderia cepacia 4-hydroxybenzoate + NADPH + 2 H+
-
?
4-hydroxybenzaldehyde + NADP+ + H2O 77% relative activity compared to vanillin Micrococcus sp. 4-hydroxybenzoate + NADPH + 2 H+
-
?
4-hydroxybenzaldehyde + NADP+ + H2O 77% relative activity compared to vanillin Micrococcus sp. TA1 4-hydroxybenzoate + NADPH + 2 H+
-
?
4-hydroxybenzaldehyde + NADP+ + H2O 70% relative activity compared to vanillin Burkholderia cepacia TM1 4-hydroxybenzoate + NADPH + 2 H+
-
?
isovanillin + NADP+ + H2O 285% relative activity compared to vanillin Micrococcus sp. isovanillate + NADPH + 2 H+
-
?
isovanillin + NADP+ + H2O 48% relative activity compared to vanillin Burkholderia cepacia isovanillate + NADPH + 2 H+
-
?
isovanillin + NADP+ + H2O 285% relative activity compared to vanillin Micrococcus sp. TA1 isovanillate + NADPH + 2 H+
-
?
isovanillin + NADP+ + H2O 48% relative activity compared to vanillin Burkholderia cepacia TM1 isovanillate + NADPH + 2 H+
-
?
protocatechualdehyde + NADP+ + H2O 146% relative activity compared to vanillin Micrococcus sp. protocatechuate + NADPH + 2 H+
-
?
protocatechualdehyde + NADP+ + H2O 94% relative activity compared to vanillin Burkholderia cepacia protocatechuate + NADPH + 2 H+
-
?
protocatechualdehyde + NADP+ + H2O 146% relative activity compared to vanillin Micrococcus sp. TA1 protocatechuate + NADPH + 2 H+
-
?
protocatechualdehyde + NADP+ + H2O 94% relative activity compared to vanillin Burkholderia cepacia TM1 protocatechuate + NADPH + 2 H+
-
?
syringaldehyde + NADP+ + H2O 23% relative activity compared to vanillin Micrococcus sp. syringate + NADPH + 2 H+
-
?
syringaldehyde + NADP+ + H2O 29% relative activity compared to vanillin Burkholderia cepacia syringate + NADPH + 2 H+
-
?
syringaldehyde + NADP+ + H2O 23% relative activity compared to vanillin Micrococcus sp. TA1 syringate + NADPH + 2 H+
-
?
syringaldehyde + NADP+ + H2O 29% relative activity compared to vanillin Burkholderia cepacia TM1 syringate + NADPH + 2 H+
-
?
vanillin + NAD+ + H2O higher activity with NAD+ than with NADP+, relative activity with NADP1 is approximately 10% Burkholderia cepacia vanillate + NADH + 2 H+
-
?
vanillin + NAD+ + H2O higher activity with NAD+ than with NADP+, relative activity with NADP1 is approximately 10% Burkholderia cepacia TM1 vanillate + NADH + 2 H+
-
?
vanillin + NADP+ + H2O no activity with NAD+, strain TA1 exhibits oxidation activity specifically under alkaline conditions Micrococcus sp. vanillate + NADPH + 2 H+
-
?
vanillin + NADP+ + H2O no activity with NAD+, strain TA1 exhibits oxidation activity specifically under alkaline conditions Micrococcus sp. TA1 vanillate + NADPH + 2 H+
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 57000, SDS-PAGE Burkholderia cepacia
homotetramer 4 * 57000, SDS-PAGE Micrococcus sp.

Synonyms

Synonyms Comment Organism
VDH
-
Burkholderia cepacia
VDH
-
Micrococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Micrococcus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 60 high activity across a wide range of temperature Burkholderia cepacia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
stable up to 30 min Micrococcus sp.
35
-
stable up to 30 min Burkholderia cepacia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 10
-
Burkholderia cepacia
9 10
-
Micrococcus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 30-min incubation at 30°C Burkholderia cepacia
7 8 30 min incubation at 30°C Micrococcus sp.