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Literature summary for 1.2.1.67 extracted from

  • Chen, H.P.; Chow, M.; Liu, C.C.; Lau, A.; Liu, J.; Eltis, L.D.
    Vanillin catabolism in Rhodococcus jostii RHA1 (2012), Appl. Environ. Microbiol., 78, 586-588.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
cloned into the pET-28a expression vector and transformed into Escherichia coli Rhodococcus jostii

Organism

Organism UniProt Comment Textmining
Rhodococcus jostii Q0SCE7
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Purification (Commentary)

Purification (Comment) Organism
partially purified by (NH4)2SO4 precipitation but it losts activity during attempted chromatographic steps Rhodococcus jostii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
vanillin + NAD+ + H2O vdh mutant Rhodococcus jostii RHA045 is unable to grow on 1 mM vanillin as the sole organic substrate but grows on 1 mM vanillate with kinetics similar to that of RHA1, mutant rescue by vdh harbouring pTipvdh plasmid Rhodococcus jostii vanillate + NADH + 2 H+
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Synonyms

Synonyms Comment Organism
ro02986
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Rhodococcus jostii
vanillin dehydrogenase
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Rhodococcus jostii
VDH
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Rhodococcus jostii

General Information

General Information Comment Organism
metabolism enzyme is involved in lignin degradation because one degradation product of lignin is vanillin Rhodococcus jostii