BRENDA - Enzyme Database show
show all sequences of 1.2.1.50

Purification of a jojoba embryo fatty acyl-coenzyme A reductase and expression of its cDNA in high erucic acid rapeseed

Metz, J.G.; Pollard, M.R.; Anderson, L.; Hayes, T.R.; Lassner, M.W.; Plant Physiol. 122, 635-644 (2000)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Simmondsia chinensis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Simmondsia chinensis
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56200
-
x * 56200, SDS-PAGE
Simmondsia chinensis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Simmondsia chinensis
-
jojoba
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
tetraeicos-cis-15-enoyl-CoA + NADPH
-
288216
Simmondsia chinensis
tetraeicos-cis-15-en-1-ol + CoA + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
x * 56200, SDS-PAGE
Simmondsia chinensis
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Simmondsia chinensis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Simmondsia chinensis
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56200
-
x * 56200, SDS-PAGE
Simmondsia chinensis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
tetraeicos-cis-15-enoyl-CoA + NADPH
-
288216
Simmondsia chinensis
tetraeicos-cis-15-en-1-ol + CoA + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
x * 56200, SDS-PAGE
Simmondsia chinensis
Other publictions for EC 1.2.1.50
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724299
Willis
Characterization of a fatty ac ...
Marinobacter hydrocarbonoclasticus
Biochemistry
50
10550-10558
2011
-
-
1
-
-
-
-
-
-
-
1
-
-
1
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-
1
-
-
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10
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1
1
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1
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1
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1
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10
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1
1
-
-
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-
-
-
-
-
725007
Hofvander
A prokaryotic acyl-CoA reducta ...
Marinobacter hydrocarbonoclasticus
FEBS Lett.
585
3538-3543
2011
-
-
1
-
-
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-
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-
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5
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1
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5
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9
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1
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5
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9
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724655
Zhu
The reductase domain in a Type ...
Cryptosporidium parvum
BMC Biochem.
11
46
2010
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1
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1
1
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725132
Teerawanichpan
A fatty acyl-CoA reductase hig ...
Apis mellifera
Insect Biochem. Mol. Biol.
40
641-649
2010
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1
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1
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7
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2
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2
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1
1
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1
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1
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1
1
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1
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2
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2
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1
1
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1
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1
1
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699807
Doan
Functional expression of five ...
Arabidopsis thaliana
J. Plant Physiol.
166
787-796
2009
-
-
1
-
-
-
-
-
-
-
5
5
-
5
-
-
-
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-
3
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5
-
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5
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5
5
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15
-
-
5
-
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-
-
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-
-
-
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-
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-
-
-
671446
Kalscheuer
Neutral lipid biosynthesis in ...
Simmondsia chinensis
Appl. Environ. Microbiol.
72
1373-1379
2006
-
1
1
-
-
-
-
-
-
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1
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1
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1
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1
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288215
Ishige
Wax ester production from n-al ...
Acinetobacter sp., Acinetobacter sp. M-1
Appl. Environ. Microbiol.
68
1192-1195
2002
-
-
1
-
-
-
-
-
1
-
1
1
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6
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12
2
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1
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2
1
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1
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2
1
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12
2
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288216
Metz
Purification of a jojoba embry ...
Simmondsia chinensis
Plant Physiol.
122
635-644
2000
-
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1
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1
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1
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1
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1
1
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1
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288212
Lee
Cysteine-286 as the site of ac ...
Photobacterium leiognathi, Photobacterium phosphoreum
Biochim. Biophys. Acta
1338
215-222
1997
-
-
2
-
6
-
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2
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6
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2
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2
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6
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2
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2
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288213
Vioque
Resolution and purification of ...
Pisum sativum
Arch. Biochem. Biophys.
340
64-72
1997
-
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-
-
-
-
-
-
1
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4
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1
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1
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1
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288211
Wang
Solubilization, purification a ...
Anas platyrhynchos
Biochem. Biophys. Res. Commun.
208
210-215
1995
1
-
-
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2
1
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1
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1
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1
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1
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1
-
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288265
Wall
Covalent reaction of cerulenin ...
Photobacterium phosphoreum
Biochem. Cell Biol.
67
163-167
1989
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1
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666
Rodriguez
-
Different sites for fatty acid ...
Photobacterium phosphoreum
Biochim. Biophys. Acta
964
266-275
1988
-
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3
1
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1
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1
1
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662
Wall
-
Subunit structure of the fatty ...
Photobacterium phosphoreum
Biochemistry
25
4315-4321
1986
-
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-
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2
1
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1
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1
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1
1
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1
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1
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1
1
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288268
Rodriguez
-
Fatty acid reductase from Phot ...
Photobacterium phosphoreum
Methods Enzymol.
133
172-182
1986
-
-
-
-
-
1
-
2
-
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3
1
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1
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1
-
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1
1
1
1
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2
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2
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1
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2
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3
1
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1
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1
1
1
1
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288269
Wall
Intersubunit transfer of fatty ...
Photobacterium phosphoreum
J. Biol. Chem.
261
15981-15988
1986
-
-
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1
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1
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1
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1
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1
1
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288270
Rodriguez
Purification of the acyl coenz ...
Photobacterium phosphoreum
J. Biol. Chem.
258
5233-5237
1983
-
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2
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1
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2
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1
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1
1
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1
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1
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1
1
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-
665
Riendeau
Resolution of the fatty acid r ...
Photobacterium phosphoreum
J. Biol. Chem.
257
6908-6915
1982
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288272
Riendeau
Fatty acid reductase in biolum ...
Photobacterium phosphoreum
Can. J. Biochem.
59
440-446
1981
-
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1
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1
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1
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