Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain TB1 | Marinobacter nauticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
hexadecanoyl-CoA | at pH 7.0 and 37°C | Marinobacter nauticus | |
0.05 | - |
cis-11-hexadecenal | at pH 7.0 and 37°C | Marinobacter nauticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
116000 | - |
x * 116000, SDS-PAGE | Marinobacter nauticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonoyl-CoA + NADPH + H+ | Marinobacter nauticus | 86% activity compared to hexadecanoyl-CoA | arachidonic aldehyde + CoA + NADP+ | - |
? | |
cis-11-hexadecenal + CoA + NADP+ | Marinobacter nauticus | - |
(E)-hexadec-11-enoyl-CoA + NADPh + H+ | - |
? | |
dodecanoyl-CoA + NADPH + H+ | Marinobacter nauticus | 26% activity compared to hexadecanoyl-CoA | dodecanal + CoA + NADP+ | - |
? | |
hexadecanoyl-CoA + NADPH + H+ | Marinobacter nauticus | 100% activity | 1-hexadecanal + CoA + NADP+ | - |
? | |
additional information | Marinobacter nauticus | the enzyme exhibits no detectable reduction with free fatty acids, less than 1% activity with acetaldehyde and propanal | ? | - |
? | |
octadecanoyl-CoA + NADPH + H+ | Marinobacter nauticus | 69% activity compared to hexadecanoyl-CoA | octadecanal + CoA + NADP+ | - |
? | |
octanoyl-CoA + NADPH + H+ | Marinobacter nauticus | 9% activity compared to hexadecanoyl-CoA | octanal + CoA + NADP+ | - |
? | |
oleoyl-CoA + NADPH + H+ | Marinobacter nauticus | 78% activity compared to hexadecanoyl-CoA | oleic aldehyde + CoA + NADP+ | - |
? | |
palmitoleoyl-CoA + NADPH + H+ | Marinobacter nauticus | 99% activity compared to hexadecanoyl-CoA | palmitoleic aldehyde + CoA + NADP+ | - |
? | |
tetradecanoyl-CoA + NADPH + H+ | Marinobacter nauticus | 60% activity compared to hexadecanoyl-CoA | tetradecanal + CoA + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Marinobacter nauticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
amylose column chromatography, metal affinity column chromatography, and G25 Sephadex gel filtration | Marinobacter nauticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.015 | - |
using octanoyl-CoA as substrate, at pH 7.0 and 37°C | Marinobacter nauticus |
0.034 | - |
using dodecanoyl-CoA as substrate, at pH 7.0 and 37°C | Marinobacter nauticus |
0.04 | - |
using octadecanoyl-CoA as substrate, at pH 7.0 and 37°C | Marinobacter nauticus |
0.044 | - |
using oleoyl-CoA as substrate, at pH 7.0 and 37°C | Marinobacter nauticus |
0.049 | - |
using arachidonoyl-CoA as substrate, at pH 7.0 and 37°C | Marinobacter nauticus |
0.057 | - |
using hexadecanoyl-CoA as substrate, at pH 7.0 and 37°C | Marinobacter nauticus |
0.057 | - |
using palmitoleyl-CoA as substrate, at pH 7.0 and 37°C | Marinobacter nauticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonoyl-CoA + NADPH + H+ | 86% activity compared to hexadecanoyl-CoA | Marinobacter nauticus | arachidonic aldehyde + CoA + NADP+ | - |
? | |
cis-11-hexadecenal + CoA + NADP+ | - |
Marinobacter nauticus | (E)-hexadec-11-enoyl-CoA + NADPh + H+ | - |
? | |
dodecanoyl-CoA + NADPH + H+ | 26% activity compared to hexadecanoyl-CoA | Marinobacter nauticus | dodecanal + CoA + NADP+ | - |
? | |
hexadecanoyl-CoA + NADPH + H+ | 100% activity | Marinobacter nauticus | 1-hexadecanal + CoA + NADP+ | - |
? | |
additional information | the enzyme exhibits no detectable reduction with free fatty acids, less than 1% activity with acetaldehyde and propanal | Marinobacter nauticus | ? | - |
? | |
octadecanoyl-CoA + NADPH + H+ | 69% activity compared to hexadecanoyl-CoA | Marinobacter nauticus | octadecanal + CoA + NADP+ | - |
? | |
octanoyl-CoA + NADPH + H+ | 9% activity compared to hexadecanoyl-CoA | Marinobacter nauticus | octanal + CoA + NADP+ | - |
? | |
oleoyl-CoA + NADPH + H+ | 78% activity compared to hexadecanoyl-CoA | Marinobacter nauticus | oleic aldehyde + CoA + NADP+ | - |
? | |
palmitoleoyl-CoA + NADPH + H+ | 99% activity compared to hexadecanoyl-CoA | Marinobacter nauticus | palmitoleic aldehyde + CoA + NADP+ | - |
? | |
tetradecanoyl-CoA + NADPH + H+ | 60% activity compared to hexadecanoyl-CoA | Marinobacter nauticus | tetradecanal + CoA + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 116000, SDS-PAGE | Marinobacter nauticus |
Synonyms | Comment | Organism |
---|---|---|
FACoAR | - |
Marinobacter nauticus |
fatty acyl-CoA reductase | - |
Marinobacter nauticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Marinobacter nauticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | optimal activity is at pH 7.0, with activity falling dramatically below pH 6.5 and above 9.0 | Marinobacter nauticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Marinobacter nauticus |