Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CRBP | enzyme recognizes retinal as substrate in presence of CRBP | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
apo-CRBP | IC50 0.0014 mM | Rattus norvegicus | |
citral | IC50 about 0.001 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
benzaldehyde | - |
Rattus norvegicus | |
0.0003 | - |
hexanal | - |
Rattus norvegicus | |
0.0003 | - |
decanal | - |
Rattus norvegicus | |
0.0003 | - |
octanal | - |
Rattus norvegicus | |
0.0017 | - |
all-trans-retinal | - |
Rattus norvegicus | |
0.0066 | - |
propanal | - |
Rattus norvegicus | |
0.022 | - |
NAD+ | standard deviation 0.004 mM | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
retinal + NAD+ + H2O | Rattus norvegicus | - |
retinoate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
-70°C, 20 mM Hepes, 2 mM DTT, 150 mM KCl, 15% glycerol, pH 8 | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzaldehyde + NAD+ + H2O | - |
Rattus norvegicus | benzoate + NADH + H+ | - |
? | |
decanal + NAD+ + H2O | - |
Rattus norvegicus | decanoic acid + NADH | - |
? | |
hexanal + NAD+ + H2O | - |
Rattus norvegicus | hexanoate + NADH + H+ | - |
? | |
octanal + NAD+ + H2O | - |
Rattus norvegicus | octanoate + NADH | - |
? | |
propanal + NAD+ + H2O | - |
Rattus norvegicus | propanoate + NADH + H+ | - |
? | |
retinal + NAD+ + H2O | - |
Rattus norvegicus | retinoate + NADH | - |
? |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | exhibits a broad pH optimum | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | reaction is NAD+ dependent, rather than NADP+ dependent | Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.001 | - |
IC50 about 0.001 mM | Rattus norvegicus | citral | |
0.0014 | - |
IC50 0.0014 mM | Rattus norvegicus | apo-CRBP |