Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.1.32 extracted from

  • Shaw, J.P.; Schwager, F.; Harayama, S.
    Substrate-specificity of benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase encoded by TOL plasmid pWW0 (1992), Biochem. J., 283, 789-794.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.32

Inhibitors

Inhibitors Comment Organism Structure
2-Chlorobenzaldehyde competitive Pseudomonas putida
2-Methoxybenzaldehyde competitive Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 3-Fluorobenzaldehyde
-
 Pseudomonas putida
0.31
-
 4-nitrobenzaldehyde
-
 Pseudomonas putida
0.31
-
 4-Fluorobenzaldehyde
-
 Pseudomonas putida
0.48
-
 2-nitrobenzaldehyde
-
 Pseudomonas putida
0.53
-
 4-methylbenzaldehyde
-
 Pseudomonas putida
0.63
-
 3-methylbenzaldehyde
-
 Pseudomonas putida
0.7
-
 4-Chlorobenzaldehyde
-
 Pseudomonas putida
0.81
-
 3-Nitrobenzaldehyde
-
 Pseudomonas putida
0.94
-
 3-Methoxybenzaldehyde
-
 Pseudomonas putida
1.1
-
 4-methoxybenzaldehyde
-
 Pseudomonas putida
1.3
-
 3-Chlorobenzaldehyde
-
 Pseudomonas putida
2
-
 2-Fluorobenzaldehyde
-
 Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
 carrying the TOL plasmid pWW0
-
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
 carrying the TOL plasmid pWW0
-

Purification (Commentary)

Purification (Comment) Organism
erroneously identified as benzaldehyde dehydrogenase Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-fluorobenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 2-fluorobenzoate + NADH + H+
-
 ?
2-fluorobenzaldehyde + NAD+ + H2O low activity Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 2-fluorobenzoate + NADH + H+
-
 ?
2-nitrobenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 2-nitrobenzoate + NADH + H+
-
 r
3-chlorobenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 3-chlorobenzoate + NADH
-
 ?
3-fluorobenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 3-fluorobenzoate + NADH + H+
-
 ?
3-methoxybenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 3-methoxybenzoate + NADH
-
 ?
3-methylbenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 3-methylbenzoate + NADH
-
 ?
3-nitrobenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida 3-nitrobenzoate + NADH
-
 ?
4-chlorobenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 4-chlorobenzoate + NADH
-
 ?
4-chlorobenzaldehyde + NAD+ + H2O low activity Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 4-chlorobenzoate + NADH
-
 ?
4-fluorobenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 4-fluorobenzoate + NADH + H+
-
 ?
4-fluorobenzaldehyde + NAD+ + H2O low activity Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 4-fluorobenzoate + NADH + H+
-
 ?
4-methoxybenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 4-methoxybenzoate + NADH + H+
-
 ?
4-methylbenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 4-methylbenzoate + NADH
-
 ?
4-nitrobenzaldehyde + NAD+ + H2O low activity Pseudomonas putida 4-nitrobenzoate + NADH + H+
-
 ?
additional information no reaction with 2-methylbenzaldehyde Pseudomonas putida ?
-
 ?
additional information no reaction with 2-chlorobenzaldehyde Pseudomonas putida ?
-
 ?
additional information no reaction with 2-methylbenzaldehyde Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 ?
-
 ?
additional information no reaction with 2-chlorobenzaldehyde Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas putida

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.06
-
 2-Methoxybenzaldehyde
-
 Pseudomonas putida
0.13
-
 2-Chlorobenzaldehyde
-
 Pseudomonas putida