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Literature summary for 1.2.1.31 extracted from

  • Calvert, A.F.; Rodwell, V.W.
    Metabolism of pipecolic acid in a Pseudomonas species. 3. L-alpha-aminoadipate delta-semialdehyde:nicotinamide adenine dinucleotide oxidoreductase (1966), J. Biol. Chem., 241, 409-414.
    View publication on PubMed
Search for more literature for 1.2.1.31

Inhibitors

Inhibitors Comment Organism Structure
acetaldehyde
-
 Pseudomonas sp.
alpha-aminoadipate
-
 Pseudomonas sp.
ATP 30% inhibition at 1 mM Pseudomonas sp.
Butyraldehyde
-
 Pseudomonas sp.
carbonyl reagents e.g.: hydroxylamine, KCN, in the presence of substrate Pseudomonas sp.
EDTA no inhibition observed Pseudomonas sp.
glutamate
-
 Pseudomonas sp.
HSO3- in presence of substrate Pseudomonas sp.
hydroxylamine in presence of substrate Pseudomonas sp.
imidazole 20% inhibition at 1 mM Pseudomonas sp.
KCN
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 DELTA1-piperidine 6-carboxylate
-
 Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal cofactor required Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 P2
-

Purification (Commentary)

Purification (Comment) Organism
using protamine sulfate treatment, ammonium sulfate fractionation and treatment with calcium phosphate gel Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.9
-
 after purification, gel fraction Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2,3,4,5-tetrahydropyridine-2-carboxylate + NAD+ + H2O
-
 Pseudomonas sp. L-2-aminoadipate + NADH
-
 ir
DELTA1-piperidine 6-carboxylate + NAD+ + H2O
-
 Pseudomonas sp. L-alpha-aminoadipate + NADH + H+
-
 r
L-2-aminoadipate + NADPH in presence of ATP + Mg2+ Pseudomonas sp. L-2-aminoadipate 6-semialdehyde + NADP+ + H2O high specificity for L-alpha-aminoadipate-semialdehyde ?
L-2-aminoadipate 6-semialdehyde + NAD+ + H2O
-
 Pseudomonas sp. L-2-aminoadipate + NADH
-
 ir
additional information not: glutamate delta-semialdehyde DELTA1-pyrroline-5-carboxylate Pseudomonas sp. ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
 L-alpha-aminoadipate-semialdehyde reduction Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6: about 10% of activity maximum, pH 9: about 70% of activity maximum Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
additional information a variety of NAD+ analogues serve as electron acceptor: deamino-NAD+, NADP+, thionicotinamide-NAD+ and 3-acetylpyridine-NAD+ Pseudomonas sp.
NAD+
-
 Pseudomonas sp.