Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a single ALDH3I1 mutant K06 line and a double T-DNA insertion mutant line K06/62 that is defective in representative members of Arabidopsis thaliana ALDH families 3 and 7, ALDH3I1 and ALDH7B4, by T-DNA insertion. The loss of function of ALDH3I1 and ALDH7B4 leads to a decrease of NAD(P)H, NAD(P)H/NAD(P) ratio, and an alteration of the glutathione pools. The aldh double mutant has higher glucose-6-phosphate dehydrogenase activity than the wild-type, indicating a high demand for reduced pyridine nucleotides. Mutant KO6 plants accumulate higher levels of reactive oxygen species (ROS) and malondialdehyde (MDA) than the wild-type. Moreover, the mutant has a reduced quantum yield of photosystem II and photosynthetic capacity at relatively high light intensities compared to the wild-type. The levels of the total glutathione (reduced + oxidized) and of the reduced to oxidized glutathione ratio (GSH/GSSG) are reduced by 20% and 33% in KO6/62 compared to wild-type, respectively. Phenotype, overview | Arabidopsis thaliana |
additional information | construction of of a single ALDH7B4 mutant K62 line and a double T-DNA insertion mutant that is defective in representative members of Arabidopsis thaliana ALDH families 3and 7, ALDH3I1 and ALDH7B4, respectively. The loss of function of ALDH3I1 and ALDH7B4 leads to a decrease of NAD(P)H, NAD(P)H/NAD(P) ratio, and an alteration of the glutathione pools. The aldh double mutant has higher glucose-6-phosphate dehydrogenase activity than the wild-type, indicating a high demand for reduced pyridine nucleotides. Mutant K62 plants accumulate higher levels of reactive oxygen species (ROS) and malondialdehyde (MDA) than the wild-type. Moreover, the mutant has a reduced quantum yield of photosystem II and photosynthetic capacity at relatively high light intensities compared to the wild-type. The levels of the total glutathione (reduced + oxidized) and of the reduced to oxidized glutathione ratio (GSH/GSSG) are reduced by 20% and 33% in KO6/62 compared to wild-type, respectively. Phenotype, overview | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
cytosol | - |
Arabidopsis thaliana | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8W033 | - |
- |
Arabidopsis thaliana | Q9SYG7 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
Synonyms | Comment | Organism |
---|---|---|
ALDH3I1 | - |
Arabidopsis thaliana |
ALDH7B4 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | stress-triggered induction of ALDH3I1 | up |
Arabidopsis thaliana | stress-triggered induction of ALDH7B4 | up |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of Arabidopsis thaliana ALDH family 3 | Arabidopsis thaliana |
evolution | the enzyme is a member of Arabidopsis thaliana ALDH family 7 | Arabidopsis thaliana |
malfunction | the combined disruption of ALDH3I1 and ALDH7B4 decreases the cellular NAD(P)H contents and alters the NAD(P)H/NAD(P)+ ratio. The aldh double mutant has higher glucose-6-phosphate dehydrogenase activity and a reduced quantum yield of photosystem II and photosynthetic capacity at relatively high light intensities compared to the wild-type. Mutant KO6 plants accumulate higher levels of reactive oxygen species (ROS) and malondialdehyde (MDA) than the wild-type plants. Disruption of ALDH3I1 and ALDH7B4 affects glutathione metabolism and photosynthesis | Arabidopsis thaliana |
physiological function | role of ALDHs as major contributors to the homeostasis of pyridine nucleotides in plants | Arabidopsis thaliana |