Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | gene yneI is responsible for NAD+/NADP+-SSADH activity. The gene is induced by succinate semialdehyde | Escherichia coli |
Application | Comment | Organism |
---|---|---|
additional information | yneI, responsible for NAD+/NADP+-dependent SSADH activity, plays a unique physiological role in the general nitrogen metabolism of Escherichia coli. The yneI gene has an important, but not essential, role during growth on arginine and probably has an essential function during growth on putrescine as the nitrogen source. The yneI-encoded activity functions primarily as a valve to prevent toxic accumulation of succinate semialdehyde | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
overexpressed as His-tagged YneI | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | yneI knockout mutant shows strongly inhibited growth compared to the wild-type | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
YneI protein | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli BW25113 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-tagged YneI dehydrogenase purified from cell extracts with HiTrap affinity columns | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinate semialdehyde + NAD+ + H2O | - |
Escherichia coli | succinate + NADH + H+ | - |
? | |
succinate semialdehyde + NAD+ + H2O | - |
Escherichia coli BW25113 | succinate + NADH + H+ | - |
? | |
succinate semialdehyde + NADP+ + H2O | - |
Escherichia coli | succinate + NADPH + H+ | - |
? | |
succinate semialdehyde + NADP+ + H2O | - |
Escherichia coli BW25113 | succinate + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SSADH | - |
Escherichia coli |
succinate semialdehyde dehydrogenase | - |
Escherichia coli |
YneI dehydrogenase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli | |
NADP+ | - |
Escherichia coli |