Cloned (Comment) | Organism |
---|---|
- |
Pyrococcus woesei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADP+ | - |
Pyrococcus woesei | |
1 | - |
NAD+ | - |
Pyrococcus woesei | |
40 | - |
phosphate | - |
Pyrococcus woesei | |
120 | - |
arsenate | - |
Pyrococcus woesei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus woesei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus woesei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pyrococcus woesei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate + arsenate + NADP+ | - |
Pyrococcus woesei | 3-phospho-D-glyceroyl arsenate + NADPH | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Pyrococcus woesei | 3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
D-glyceraldehyde 3-phosphate + phosphate + NADP+ | - |
Pyrococcus woesei | 3-phospho-D-glyceroyl phosphate + NADPH | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
sodium chloride, potassium chloride, sodium sulfate, potassium sulfate, sodium phosphate, potassium phosphate, sodium citrate and potassium citrate stabilize against thermal inactivation at 104°C. K+ is more effective than Na+ with respect to anion | Pyrococcus woesei |
97 | - |
half-life: 65 min | Pyrococcus woesei |
100 | - |
half-life: 44 min | Pyrococcus woesei |
104 | - |
half-life: 9 min without stabilizer, stable for 30 min in presence of 250 mM potassium citrate | Pyrococcus woesei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | cofactor | Pyrococcus woesei | |
NADP+ | cofactor | Pyrococcus woesei |