Cloned (Comment) | Organism |
---|---|
into the pET28a vector for expression in Escherichia coli BL21DE3 cells, amplificatied in Escherichia coli HB101 cells | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | DTT | Arabidopsis thaliana | |
H2O2 | - |
Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
monomer, determined by SDS-PAGE | Arabidopsis thaliana |
120000 | - |
tetramer, determined by gel filtration | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glyceroyl phosphate + NADH + H+ | Arabidopsis thaliana | - |
D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification protocol comprises two anion-exchange chromatographic steps and removing of the His tag | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
Arabidopsis thaliana | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
GapA | A4 isoform of GAPDH | Arabidopsis thaliana |
GAPDH | - |
Arabidopsis thaliana |
glyceraldehyde-3-phosphate dehydrogenase | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Arabidopsis thaliana |