Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor-diffusion method, enzyme in complex with NAD+ and D-glyceraldehyde 3-phosphate. C149A GAPDH and C149S GAPDH ternary complexes are obtained by soaking the crystals of the corresponding binary complexes (enzyme -NAD+) in a solution containing D-glyceraldehyde 3-phosphate | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
C149A | mutant enzyme displays no significant dehydrogenase activity | Geobacillus stearothermophilus |
C149S | low but significant phosphorylating dehydrogenase activity | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | P00362 | - |
- |