Protein Variants | Comment | Organism |
---|---|---|
H63A | about 17% residual activity in assay using 2,6-dichlorophenolindophenol, only modest inhibition by acetylphosphinate and acetylmethylphosphinate | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetylmethylphosphinate | partially reversible tight-binding inhibitor, formation of a C2alpha-phosphinolactylthiamine diphosphate derivative involving H63 residue of enzyme | Escherichia coli | |
acetylmethylphosphinate | partially reversible tight-binding inhibitor, formation of a C2alpha-phosphinolactylthiamine diphosphate derivative involving H63 residue of enzyme | Homo sapiens | |
Acetylphosphinate | partially reversible tight-binding inhibitor, formation of a C2alpha-phosphinolactylthiamine diphosphate derivative involving H63 residue of enzyme | Escherichia coli | |
Acetylphosphinate | partially reversible tight-binding inhibitor, formation of a C2alpha-phosphinolactylthiamine diphosphate derivative involving H63 residue of enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + acetylphosphinate + NAD+ | - |
Homo sapiens | (R)-acetoin + ? + NADH | - |
? | |
pyruvate + acetylphosphinate + NAD+ | - |
Escherichia coli | (S)-acetoin + ? + NADH | - |
? | |
pyruvate + CoA + NAD+ | - |
Escherichia coli | acetyl-CoA + CO2 + NADH | - |
? | |
pyruvate + CoA + NAD+ | - |
Homo sapiens | acetyl-CoA + CO2 + NADH | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000014 | - |
Acetylphosphinate | pH 8.0, 30°C | Homo sapiens | |
0.00076 | - |
Acetylphosphinate | pH 8.0, 30°C | Escherichia coli | |
0.00146 | - |
acetylmethylphosphinate | pH 8.0, 30°C | Homo sapiens | |
0.0033 | - |
acetylmethylphosphinate | pH 8.0, 30°C | Escherichia coli |