Cloned (Comment) | Organism |
---|---|
pT7.5-dmpFG plasmid expressed in Escherichia coli C41(DE3) | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli XL2-Blue | - |
- |
- |
Purification (Comment) | Organism |
---|---|
on DEAE and NAD+-linked affinity columns, combined with an ammonium sulfate fractionation step | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + CoA + NAD+ + H+ | - |
Escherichia coli | acetyl-CoA + NADH | - |
? | |
acetaldehyde + CoA + NAD+ + H+ | - |
Escherichia coli XL2-Blue | acetyl-CoA + NADH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DmpF | - |
Escherichia coli |
NAD+/CoA-dependent aldehyde dehydrogenase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
CoA | shares a binding site with NAD+. Rossmann fold can alternately bind CoA or NAD+ cofactors required for enzymatic catalysis | Escherichia coli | |
NAD+ | shares a binding site with CoA. Rossmann fold can alternately bind CoA or NAD+ cofactors required for enzymatic catalysis | Escherichia coli |