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Literature summary for 1.18.6.2 extracted from

  • Eady, R.; Richardson, T.; Miller, R.; Hawkins, M.; Lowe, D.
    The vanadium nitrogenase of Azotobacter chroococcum. Purification and properties of the Fe protein (1988), Biochem. J., 256, 189-196 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.18.6.2

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 4 mol of iron per mol of iron-subunit Azotobacter chroococcum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62500
-
 gel filtration, iron-subunit Azotobacter chroococcum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
12 reduced ferredoxin + 12 H+ + N2 + 40 ATP + 40 H2O Azotobacter chroococcum
-
 12 oxidized ferredoxin + 3 H2 + 2 NH3 + 40 ADP + 40 phosphate
-
 ?
12 reduced ferredoxin + 12 H+ + N2 + 40 ATP + 40 H2O Azotobacter chroococcum MCD1
-
 12 oxidized ferredoxin + 3 H2 + 2 NH3 + 40 ADP + 40 phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Azotobacter chroococcum P15332 and P15334 and P15333 P15332 i.e. subunit VnfD, P15334 i.e. subunit VnfK, P15333 i.e. subunit VnfG
-
Azotobacter chroococcum MCD1 P15332 and P15334 and P15333 P15332 i.e. subunit VnfD, P15334 i.e. subunit VnfK, P15333 i.e. subunit VnfG
-

Purification (Commentary)

Purification (Comment) Organism
purification of the iron-subint Azotobacter chroococcum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
337
-
 formation of NH3, pH not specified in the publication, temperature not specified in the publication Azotobacter chroococcum
1211
-
 evolution of H2 under argon atmosphere, pH not specified in the publication, temperature not specified in the publication Azotobacter chroococcum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
12 reduced ferredoxin + 12 H+ + N2 + 40 ATP + 40 H2O
-
 Azotobacter chroococcum 12 oxidized ferredoxin + 3 H2 + 2 NH3 + 40 ADP + 40 phosphate
-
 ?
12 reduced ferredoxin + 12 H+ + N2 + 40 ATP + 40 H2O
-
 Azotobacter chroococcum MCD1 12 oxidized ferredoxin + 3 H2 + 2 NH3 + 40 ADP + 40 phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 32000, SDS-PAGE, isolated iron-subunit Azotobacter chroococcum

Synonyms

Synonyms Comment Organism
vnfD alpha-chain Azotobacter chroococcum
vnfG delta-chain Azotobacter chroococcum
vnfK beta-chain Azotobacter chroococcum