Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Entamoeba histolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no substrate inhibition at high NAD(P)H (up to 0.3 mM) | Entamoeba histolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
oxidized Clostridium pasteurianum rubredoxin | with NADPH as cosubstrate, at pH 7.0 and 30°C | Entamoeba histolytica | |
0.0032 | - |
oxidized Pyrococcus furiosus rubredoxin | with NADPH as cosubstrate, at pH 7.0 and 30°C | Entamoeba histolytica | |
0.0043 | - |
oxidized Clostridium pasteurianum rubredoxin | with NADH as cosubstrate, at pH 7.0 and 30°C | Entamoeba histolytica | |
0.0052 | - |
oxidized Pyrococcus furiosus rubredoxin | with NADH as cosubstrate, at pH 7.0 and 30°C | Entamoeba histolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
gel filtration | Entamoeba histolytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | - |
- |
- |
Entamoeba histolytica HM1-IMSS | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap IMAC-Ni2+ column chromatography | Entamoeba histolytica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
trophozoite | - |
Entamoeba histolytica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme has no activity with ferredoxins, flavodoxins, and thioredoxins | Entamoeba histolytica | ? | - |
? | |
additional information | the enzyme has no activity with ferredoxins, flavodoxins, and thioredoxins | Entamoeba histolytica HM1-IMSS | ? | - |
? | |
oxidized Clostridium pasteurianum rubredoxin + NADH | - |
Entamoeba histolytica | reduced Clostridium pasteurianum rubredoxin + NAD+ + H+ | - |
? | |
oxidized Clostridium pasteurianum rubredoxin + NADH | - |
Entamoeba histolytica HM1-IMSS | reduced Clostridium pasteurianum rubredoxin + NAD+ + H+ | - |
? | |
oxidized Clostridium pasteurianum rubredoxin + NADPH | - |
Entamoeba histolytica | reduced Clostridium pasteurianum rubredoxin + NADP+ + H+ | - |
? | |
oxidized Clostridium pasteurianum rubredoxin + NADPH | - |
Entamoeba histolytica HM1-IMSS | reduced Clostridium pasteurianum rubredoxin + NADP+ + H+ | - |
? | |
oxidized flavodiiron protein + NADH | - |
Entamoeba histolytica | reduced flavodiiron protein + NAD+ + H+ | - |
? | |
oxidized flavodiiron protein + NADH | - |
Entamoeba histolytica HM1-IMSS | reduced flavodiiron protein + NAD+ + H+ | - |
? | |
oxidized flavodiiron protein + NADPH | - |
Entamoeba histolytica | reduced flavodiiron protein + NADP+ + H+ | - |
? | |
oxidized flavodiiron protein + NADPH | - |
Entamoeba histolytica HM1-IMSS | reduced flavodiiron protein + NADP+ + H+ | - |
? | |
oxidized Pyrococcus furiosus rubredoxin + NADH | - |
Entamoeba histolytica | reduced Pyrococcus furiosus rubredoxin + NAD+ + H+ | - |
? | |
oxidized Pyrococcus furiosus rubredoxin + NADPH | - |
Entamoeba histolytica | reduced Pyrococcus furiosus rubredoxin + NADP+ + H+ | - |
? | |
oxidized rubrerythrin + NADH | - |
Entamoeba histolytica | reduced rubrerythrin + NAD+ + H+ | - |
? | |
oxidized rubrerythrin + NADPH | - |
Entamoeba histolytica | reduced rubrerythrin + NADP+ + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE | Entamoeba histolytica |
monomer | 1 * 49700, calculated from amino acid sequence | Entamoeba histolytica |
Synonyms | Comment | Organism |
---|---|---|
NAD(P)H-dependent rubredoxin reductase | - |
Entamoeba histolytica |
NROR | - |
Entamoeba histolytica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Entamoeba histolytica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | 6 | oxidized Clostridium pasteurianum rubredoxin | with NADH as cosubstrate, at pH 7.0 and 30°C | Entamoeba histolytica | |
9.6 | - |
oxidized Pyrococcus furiosus rubredoxin | with NADPH as cosubstrate, at pH 7.0 and 30°C | Entamoeba histolytica | |
13.9 | - |
oxidized Clostridium pasteurianum rubredoxin | with NADPH as cosubstrate, at pH 7.0 and 30°C | Entamoeba histolytica | |
69.4 | - |
oxidized Pyrococcus furiosus rubredoxin | with NADH as cosubstrate, at pH 7.0 and 30°C | Entamoeba histolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Entamoeba histolytica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Entamoeba histolytica | |
NADH | the catalytic efficiency exhibited by this enzyme for NADH is higher than for NADPH | Entamoeba histolytica | |
NADPH | the catalytic efficiency exhibited by this enzyme for NADH is higher than for NADPH | Entamoeba histolytica |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Entamoeba histolytica | calculated from amino acid sequence | - |
8.2 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3000 | - |
oxidized Pyrococcus furiosus rubredoxin | with NADPH as cosubstrate, at pH 7.0 and 30°C | Entamoeba histolytica | |
5000 | - |
oxidized Clostridium pasteurianum rubredoxin | with NADPH as cosubstrate, at pH 7.0 and 30°C | Entamoeba histolytica | |
8400 | - |
oxidized Clostridium pasteurianum rubredoxin | with NADH as cosubstrate, at pH 7.0 and 30°C | Entamoeba histolytica | |
13000 | - |
oxidized Pyrococcus furiosus rubredoxin | with NADH as cosubstrate, at pH 7.0 and 30°C | Entamoeba histolytica |