Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 10 mM, 30% inhibition | Pseudomonas sp. | |
iodoacetate | 10 mM, 50% inhibition | Pseudomonas sp. | |
NEM | 10 mM, 67% inhibition | Pseudomonas sp. | |
PCMB | 0.0005 mM, 94% inhibition | Pseudomonas sp. | |
Sodium azide | 40 mM, 46% inhibition | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | iron-sulfur flavoprotein, protein contains 1.8 gatom of iron and 2.0 gatom of acid-labile sulfur | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34900 | - |
non-denaturing PAGE | Pseudomonas sp. |
36000 | - |
1 * 36000, SDS-PAGE | Pseudomonas sp. |
37000 | - |
gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. | - |
NADH-ferredoxinNAP reductase component of naphthalene dioxygenase | - |
Pseudomonas sp. NCIB 9816 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
NADH-ferredoxinNAP reductase component of naphthalene dioxygenase | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
397 | - |
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-20°C, 1 month, minimal loss of activity | Pseudomonas sp. |
0-5°C, 30% loss of activity after 5 days | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricyanide + NADH | - |
Pseudomonas sp. | 2 ferrocyanide + NAD+ + H+ | - |
? | |
2 ferricyanide + NADH | - |
Pseudomonas sp. NCIB 9816 | 2 ferrocyanide + NAD+ + H+ | - |
? | |
2 ferricytochrome c + NADH | - |
Pseudomonas sp. | 2 ferrocytochrome c + NAD+ + H+ | - |
? | |
2 ferricytochrome c + NADH | - |
Pseudomonas sp. NCIB 9816 | 2 ferrocytochrome c + NAD+ + H+ | - |
? | |
NADH + oxidized 2,6-dichlorophenolindophenol + H+ | - |
Pseudomonas sp. | NAD+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
NADH + oxidized 2,6-dichlorophenolindophenol + H+ | - |
Pseudomonas sp. NCIB 9816 | NAD+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
NADH + oxidized nitro blue tetrazolium + H+ | - |
Pseudomonas sp. | NAD+ + reduced nitro blue tetrazolium | - |
? | |
NADH + oxidized nitro blue tetrazolium + H+ | - |
Pseudomonas sp. NCIB 9816 | NAD+ + reduced nitro blue tetrazolium | - |
? | |
NADPH + oxidized cytochrome c | 39% of the activity with NADH | Pseudomonas sp. | NADP+ + reduced cytochrome c | - |
? | |
NADPH + oxidized cytochrome c | 39% of the activity with NADH | Pseudomonas sp. NCIB 9816 | NADP+ + reduced cytochrome c | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36000, SDS-PAGE | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
NADH-ferredoxinNAP reductase | component of naphthalene dioxygenase multicomponent enzyme system | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
0.5 | - |
5 d, 30% loss of activity | Pseudomonas sp. |
21 | - |
room temperature, 8 h, 50% loss of activity | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 mol of FAD is bound per mol of enzyme | Pseudomonas sp. | |
NADH | - |
Pseudomonas sp. | |
NADPH | 39% of the activity with NADH in the reaction with cytochrome c | Pseudomonas sp. |