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Literature summary for 1.17.9.1 extracted from
- The catabolism of 2,4-xylenol and p-cresol share the enzymes for the oxidation of para-methyl group in Pseudomonas putida NCIMB 9866 (2014), Appl. Microbiol. Biotechnol., 98, 1349-1356.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA-chromatography | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,4-xylenol + acceptor + H2O | - |
Pseudomonas putida | 3-methyl-4-hydroxybenzaldehyde + reduced acceptor | - |
? |  |
| 4-cresol + acceptor + H2O | - |
Pseudomonas putida | 4-hydroxybenzaldehyde + reduced acceptor | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| p-cresol methylhydroxylase | - |
Pseudomonas putida |
| pchF | - |
Pseudomonas putida |
| PCMH | - |
Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
assay at | Pseudomonas putida |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | using a gene-knockout it is shown that PCMH-encoding gene pchF is necessary for the catabolism of 2,4-xylenol | Pseudomonas putida |
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Release 2023.1 (January 2023)
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