Application | Comment | Organism |
---|---|---|
drug development | class Ib NrdF requires manganese under conditions in which the organism is pathogenic. Considering that streptococci and other pathogens, including enterococci, staphylococci, and Bacillus sp., contain class Ib ribonucleotide reductase as their only aerobic RNR, prevention of dimanganese-tyrosyl radical (Mn(III)2-Y(*)) cofactor formation in the class Ib ribonucleotide reductase may be an attractive target for new antimicrobials | Streptococcus sanguinis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) cells | Streptococcus sanguinis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | the dimanganese-tyrosyl radical (Mn(III)2-Y(*)) cofactor is 3.5fold more active than the iron form | Streptococcus sanguinis | |
Mn3+ | the enzyme uses a dimanganese-tyrosyl radical (Mn(III)2-Y(*)) cofactor in vivo | Streptococcus sanguinis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus sanguinis | A3CLZ6 AND A3CLZ4 | A3CLZ6: subunit alpha (NrdE), A3CLZ4: subunit beta (NrdF) | - |
Streptococcus sanguinis SK36 | A3CLZ6 AND A3CLZ4 | A3CLZ6: subunit alpha (NrdE), A3CLZ4: subunit beta (NrdF) | - |
Purification (Comment) | Organism |
---|---|
- |
Streptococcus sanguinis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP + thioredoxin | - |
Streptococcus sanguinis | 2'-dCDP + thioredoxin disulfide + H2O | - |
? | |
CDP + thioredoxin | - |
Streptococcus sanguinis SK36 | 2'-dCDP + thioredoxin disulfide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
class Ib ribonucleotide reductase | - |
Streptococcus sanguinis |
class Ib RNR | - |
Streptococcus sanguinis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Streptococcus sanguinis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
diferric(III)-tyrosyl radical cofactor | the dimanganese-tyrosyl radical (Mn(III)2-Y(*)) cofactor is 3.5fold more active than the iron form. The flavodoxin NrdI is essential for assembly of the ribonucleotide reductase metallo-cofactor | Streptococcus sanguinis | |
dimanganese-tyrosyl radical cofactor | the enzyme uses a dimanganese-tyrosyl radical (Mn(III)2-Y(*)) cofactor in vivo. The dimanganese-tyrosyl radical (Mn(III)2-Y(*)) cofactor is 3.5fold more active than the iron form. The flavodoxin NrdI is essential for assembly of the ribonucleotide reductase metallo-cofactor | Streptococcus sanguinis |