Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dATP | activation at low concentration with a KL1 value for specificity site binding of 0.0032 mM, inhibition at higher concentration with a KL2 value for activity site binding of 0.0173 mM | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | truncated NrdA lacking the N-terminal ATP-cone forms an NrdA2NrdB2 complex. Mutant protein is completely resistant to high concentrations of dATP | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dATP | activation at low concentration with a KL1 value for specificity site binding of 0.0032 mM, inhibition at higher concentration with a KL2 value for activity site binding of 0.0173 mM | Pseudomonas aeruginosa | |
additional information | not inhibitory: EDTA | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | iron is bound tightly to the protein. Enzyme activity is the same in presence and absence of EDTA | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
4 * 107000, subunit NrdA + 4 * 47000, subunit NrdB, SDS-PAGE | Pseudomonas aeruginosa |
107000 | - |
4 * 107000, subunit NrdA + 4 * 47000, subunit NrdB, SDS-PAGE | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
enzyme is a complex of the NrdA protein harboring the active site and the allosteric sites and the NrdB protein harboring a tyrosyl radical | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
22 | - |
pH 7.5, 25°C, anaerobic incubation and assay | Pseudomonas aeruginosa |
66 | - |
pH 7.5, 25°C, anaerobic incubation, aerobic assay | Pseudomonas aeruginosa |
116 | - |
pH 7.5, 25°C, aerobic incubation and assay | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP + reduced dithiothreitol | - |
Pseudomonas aeruginosa | 2'-dCDP + oxidized dithiothreitol + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme is a complex of the NrdA protein harboring the active site and the allosteric sites and the NrdB protein harboring a tyrosyl radical. Wild-type enzyme consists of four NrdA and four NrdB subunits. A truncated NrdA lacking the N-terminal ATP-cone forms an NrdA2NrdB2 complex | Pseudomonas aeruginosa |
octamer | 4 * 107000, subunit NrdA + 4 * 47000, subunit NrdB, SDS-PAGE | Pseudomonas aeruginosa |