Cloned (Comment) | Organism |
---|---|
expression of wild-type and Y177F mutant subunit R2 in Escherichia coli | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
Y177F | tyrosyl residue involved in radical formation | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the essential metallo-cofactor is a micro-oxo-micro-carboxylato-diiron cluster adjacent to a stable tyrosyl radical | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2'-deoxyribonucleoside 5'-diphosphate + thioredoxin disulfide + H2O = ribonucleoside 5'-diphosphate + thioredoxin | kinetics and mechanism of formation of the tyrosyl radical and micro-oxo-diiron cluster in the R2 subunit | Mus musculus |