Activating Compound | Comment | Organism | Structure |
---|---|---|---|
adenyl-5'-yl-imidodiphosphate | stimulation at low concentration, inhibition above 0.3 mM | Vaccinia virus | |
ATP | most effective activator | Vaccinia virus | |
P1,P4-bis(5'-adenosyl) tetraphosphate | stimulation at low concentrations, inhibition above 0.3 mM | Vaccinia virus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Vaccinia virus |
General Stability | Organism |
---|---|
quite susceptible to denaturation | Vaccinia virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 2fold activation | Vaccinia virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vaccinia virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Blue Sepharose, affinity resin | Vaccinia virus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.122 | - |
recombinant enzyme | Vaccinia virus |
Storage Stability | Organism |
---|---|
-80°C, several months, no loss of activity | Vaccinia virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ribonucleoside diphosphate + reduced thioredoxin | dithiothreitol serves as in vitro electron donor, maximal activity with 50-75 mM | Vaccinia virus | 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O | - |
ir |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.8 | - |
Vaccinia virus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8.8 | 60% activity at pH 7.2 | Vaccinia virus |