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Literature summary for 1.17.1.9 extracted from

  • Jaroensuk, J.; Intasian, P.; Kiattisewee, C.; Munkajohnpon, P.; Chunthaboon, P.; Buttranon, S.; Trisrivirat, D.; Wongnate, T.; Maenpuen, S.; Tinikul, R.; Chaiyen, P.
    Addition of formate dehydrogenase increases the production of renewable alkane from an engineered metabolic pathway (2019), J. Biol. Chem., 294, 11536-11548 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Xanthobacter sp. 91

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 NAD+ at pH 7.0 and 25°C Xanthobacter sp. 91
9
-
 formate with NAD+ as cosubstrate, at pH 7.0 and 25°C Xanthobacter sp. 91
37
-
 NADP+ at pH 7.0 and 25°C Xanthobacter sp. 91
100
-
 formate with NADP+ as cosubstrate, at pH 7.0 and 25°C Xanthobacter sp. 91

Organism

Organism UniProt Comment Textmining
Xanthobacter sp. 91
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
 Xanthobacter sp. 91 CO2 + NADH + H+
-
 ?
formate + NADP+
-
 Xanthobacter sp. 91 CO2 + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
FDH
-
 Xanthobacter sp. 91

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Xanthobacter sp. 91

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
 NADP+ at pH 7.0 and 25°C Xanthobacter sp. 91
0.4
-
 formate with NADP+ as cosubstrate, at pH 7.0 and 25°C Xanthobacter sp. 91
31
-
 NAD+ at pH 7.0 and 25°C Xanthobacter sp. 91
31
-
 formate with NAD+ as cosubstrate, at pH 7.0 and 25°C Xanthobacter sp. 91

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 the enzyme activity decreases upon pH increases Xanthobacter sp. 91

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme prefers to use NAD+ as an electron acceptor more than NADP+ Xanthobacter sp. 91
NADP+ the enzyme prefers to use NAD+ as an electron acceptor more than NADP+ Xanthobacter sp. 91

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0019
-
 formate with NADP+ as cosubstrate, at pH 7.0 and 25°C Xanthobacter sp. 91
0.011
-
 NADP+ at pH 7.0 and 25°C Xanthobacter sp. 91
3
-
 formate with NAD+ as cosubstrate, at pH 7.0 and 25°C Xanthobacter sp. 91
479
-
 NAD+ at pH 7.0 and 25°C Xanthobacter sp. 91