Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) pLysS cells | [Candida] boidinii |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | [Candida] boidinii |
Protein Variants | Comment | Organism |
---|---|---|
K328V | the mutant shows increased catalytic efficiency for NAD+ compared to the wild type enzyme | [Candida] boidinii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | nanomolar inhibitor | [Candida] boidinii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
NAD+ | mutant enzyme K328V, at pH 7.5 and 25°C | [Candida] boidinii | |
0.0303 | - |
NAD+ | recombinant enzyme, at pH 7.5 and 25°C | [Candida] boidinii | |
4 | - |
formate | recombinant enzyme, at pH 7.5 and 25°C | [Candida] boidinii | |
5 | - |
formate | mutant enzyme K328V, at pH 7.5 and 25°C | [Candida] boidinii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | [Candida] boidinii | - |
CO2 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Candida] boidinii | O13437 | - |
- |
Purification (Comment) | Organism |
---|---|
Blue Sepharose 6 column chromatography and Superdex 200 gel filtration | [Candida] boidinii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | - |
[Candida] boidinii | CO2 + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FDH | - |
[Candida] boidinii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.6 | - |
NAD+ | recombinant enzyme, at pH 7.5 and 25°C | [Candida] boidinii | |
5.6 | - |
formate | recombinant enzyme, at pH 7.5 and 25°C | [Candida] boidinii | |
6.8 | - |
NAD+ | mutant enzyme K328V, at pH 7.5 and 25°C | [Candida] boidinii | |
6.8 | - |
formate | mutant enzyme K328V, at pH 7.5 and 25°C | [Candida] boidinii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
[Candida] boidinii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
formate | recombinant enzyme, at pH 7.5 and 25°C | [Candida] boidinii | |
1.4 | - |
formate | mutant enzyme K328V, at pH 7.5 and 25°C | [Candida] boidinii | |
180 | - |
NAD+ | recombinant enzyme, at pH 7.5 and 25°C | [Candida] boidinii | |
270 | - |
NAD+ | mutant enzyme K328V, at pH 7.5 and 25°C | [Candida] boidinii |