Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thiobacillus sp. |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.1 M HEPES pH 7.0, 25% (w/v) polyethylene glycol 6000, 1.2 M lithium chloride | Thiobacillus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | Thiobacillus sp. | - |
CO2 + NADH + H+ | - |
? | |
formate + NAD+ | Thiobacillus sp. KNK65MA | - |
CO2 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thiobacillus sp. | Q76EB7 | - |
- |
Thiobacillus sp. KNK65MA | Q76EB7 | - |
- |
Purification (Comment) | Organism |
---|---|
glutathione Sepharose column chromatography and Superdex 200 gel filtration | Thiobacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | - |
Thiobacillus sp. | CO2 + NADH + H+ | - |
? | |
formate + NAD+ | - |
Thiobacillus sp. KNK65MA | CO2 + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NAD-dependent FDH | - |
Thiobacillus sp. |
NAD-dependent formate dehydrogenase | - |
Thiobacillus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thiobacillus sp. |