Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Ogataea angusta |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM Cu2+ inhibits the enzyme activity by 79% | Ogataea angusta | |
Ni2+ | 1 mM Ni2+ inhibits the enzyme activity by 28% | Ogataea angusta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.083 | - |
NAD+ | at pH 6.5 and 30°C | Ogataea angusta | |
0.82 | - |
formate | at pH 6.5 and 30°C | Ogataea angusta |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM Ca2+ improves the enzyme activity by 77% | Ogataea angusta | |
Co2+ | 1 mM Co2+ improves the enzyme activity by 46% | Ogataea angusta | |
additional information | Mn2+, Ba2+, and Mg2+ do not significantly change the enzyme activity | Ogataea angusta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39994 | - |
2 * 39994, calculated from amino acid sequence | Ogataea angusta |
40000 | - |
2 * 40000, SDS-PAGE | Ogataea angusta |
78000 | - |
native enzyme, gel filtration | Ogataea angusta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | Ogataea angusta | - |
CO2 + NADH + H+ | - |
? | |
formate + NAD+ | Ogataea angusta DL-1 | - |
CO2 + NADH + H+ | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
dimethyl sulfoxide | the enzyme activity almost does not decrease after incubation in 20% (v/v) dimethyl sulfoxide | Ogataea angusta |
n-Butanol | the enzyme activity almost does not decrease after incubation in 20% (v/v) n-butanol | Ogataea angusta |
n-hexane | the enzyme activity almost does not decrease after incubation in 20% (v/v) n-hexane | Ogataea angusta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ogataea angusta | P33677 | formerly Ogataea parapolymorpha | - |
Ogataea angusta DL-1 | P33677 | formerly Ogataea parapolymorpha | - |
Purification (Comment) | Organism |
---|---|
nickel chelating Sepharose column chromatography | Ogataea angusta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | - |
Ogataea angusta | CO2 + NADH + H+ | - |
? | |
formate + NAD+ | - |
Ogataea angusta DL-1 | CO2 + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 40000, SDS-PAGE | Ogataea angusta |
homodimer | 2 * 39994, calculated from amino acid sequence | Ogataea angusta |
Synonyms | Comment | Organism |
---|---|---|
FDH | - |
Ogataea angusta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Ogataea angusta |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | the enzyme has more than 70% of relative activity when the temperature is between 60 and 70°C, but the enzyme activity decreases abruptly when the temperature is controlled below 60°C or above 70 °C | Ogataea angusta |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | the enzyme retains 93, 88, 83, and 71% of its initial activity after 4 h of exposure at 40, 50, 55, and 60 °C, respectively | Ogataea angusta |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.32 | - |
formate | at pH 6.5 and 30°C | Ogataea angusta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | - |
Ogataea angusta |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | the enzyme displays a relatively wide pH spectrum and shows more than 98% of relative activity from pH 6.0 to 7.0 and still has 81 and 75% of relative activity under pH 7.5 and 8.0, respectively | Ogataea angusta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Ogataea angusta |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.83 | - |
formate | at pH 6.5 and 30°C | Ogataea angusta |