Protein Variants | Comment | Organism |
---|---|---|
D196A/Y197R | coenzyme preference is shifted from NAD+ to NADP+, decreased thermal stability at 42°C | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0365 | - |
NAD+ | pH 7.0, 30°C, wild-type enzyme | Saccharomyces cerevisiae | |
0.13 | - |
NADP+ | pH 7.0, 30°C, mutant enzyme D196A/Y197R, with 0.25 M formate | Saccharomyces cerevisiae | |
0.16 | - |
NADP+ | pH 7.0, 30°C, mutant enzyme D196A/Y197R, with 0.5 M formate | Saccharomyces cerevisiae | |
5.5 | - |
formate | pH 7.0, 30°C, wild-type enzyme | Saccharomyces cerevisiae | |
7.6 | - |
NAD+ | pH 7.0, 30°C, mutant enzyme D196A/Y197R, with 0.25 M formate | Saccharomyces cerevisiae | |
8.4 | - |
NAD+ | pH 7.0, 30°C, mutant enzyme D196A/Y197R, with 0.5 M formate | Saccharomyces cerevisiae | |
1000 | - |
formate | pH 7.0, 30°C, mutant enzyme D196A/Y197R, reaction with NADP+ | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q08911 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | wild-type enzyme shows no activity with NADP+, mutant enzyme D196A/Y197R shows higher activity with NADP+ than with NAD+ | Saccharomyces cerevisiae | CO2 + NADH + H+ | - |
? | |
formate + NADP+ | wild-type enzyme shows no activity, mutant enzyme D196A/Y197R shows higher activity with NADP+ than with NAD+ | Saccharomyces cerevisiae | CO2 + NADPH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SceFDH | - |
Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
wild-type enzyme loses 50% of its activity after 3.5 min, mutant enzyme D196A/Y197R loses 50% of its activity after 1.5 min | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.095 | - |
NAD+ | pH 7.0, 30°C, mutant enzyme D196A/Y197R | Saccharomyces cerevisiae | |
0.12 | - |
NAD+ | pH 7.0, 30°C, mutant enzyme D196A/Y197R, with 0.5 M formate | Saccharomyces cerevisiae | |
4.5 | - |
NADP+ | pH 7.0, 30°C, mutant enzyme D196A/Y197R | Saccharomyces cerevisiae | |
6.5 | - |
NAD+ | pH 7.0, 30°C, wild-type enzyme | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | wild-type enzyme shows no activity with NADP+, mutant enzyme D196A/Y197R shows higher activity with NADP+ than with NAD+ | Saccharomyces cerevisiae | |
NADP+ | wild-type enzyme shows no activity with NADP+, mutant enzyme D196A/Y197R shows higher activity with NADP+ than with NAD+ | Saccharomyces cerevisiae |