General Stability | Organism |
---|---|
N3- and NO3- stabilize | Cupriavidus necator |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CN- | - |
Cupriavidus necator | |
Hg2+ | - |
Cupriavidus necator | |
N3- | - |
Cupriavidus necator | |
NO3- | 10 mM KNO3, 38% inhibition | Cupriavidus necator |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
NAD+ | - |
Cupriavidus necator | |
3.3 | - |
formate | - |
Cupriavidus necator |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Molybdenum | 0.64 gatom per mol of enzyme. The enzyme contains molybdopterin guanine dinucleotide | Cupriavidus necator |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
11600 | - |
alpha,beta,gamma,delta, 1 * 110000 + 1 * 57000 + 1 * 19400 + 1 * 11600, SDS-PAGE | Cupriavidus necator |
19400 | - |
alpha,beta,gamma,delta, 1 * 110000 + 1 * 57000 + 1 * 19400 + 1 * 11600, SDS-PAGE | Cupriavidus necator |
57000 | - |
alpha,beta,gamma,delta, 1 * 110000 + 1 * 57000 + 1 * 19400 + 1 * 11600, SDS-PAGE | Cupriavidus necator |
110000 | - |
alpha,beta,gamma,delta, 1 * 110000 + 1 * 57000 + 1 * 19400 + 1 * 11600, SDS-PAGE | Cupriavidus necator |
197000 | - |
non-denaturing gradient PAGE | Cupriavidus necator |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Cupriavidus necator |
Storage Stability | Organism |
---|---|
-20°C, stable for several weeks | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + 2,6-dichloroindophenol | 54% of the activity with NAD+ | Cupriavidus necator | CO2 + reduced 2,6-dichlorophenolindophenol | - |
? | |
formate + benzyl viologen | 113% of the activity with NAD+ | Cupriavidus necator | CO2 + reduced benzyl viologen | - |
? | |
formate + ferricyanide | 10% of the activity with NAD+ | Cupriavidus necator | CO2 + ferrocyanide | - |
? | |
formate + methyl viologen | 74% of the activity with NAD+ | Cupriavidus necator | CO2 + reduced methyl viologen | - |
? | |
formate + methylene blue | 119% of the activity with NAD+ | Cupriavidus necator | CO2 + reduced methylene blue | - |
? | |
formate + NAD+ | - |
Cupriavidus necator | CO2 + NADH + H+ | - |
? | |
formate + nitrobluetetrazolium | 54% of the activity with NAD+ | Cupriavidus necator | CO2 + reduced nitrobluetetrazolium | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha,beta,gamma,delta, 1 * 110000 + 1 * 57000 + 1 * 19400 + 1 * 11600, SDS-PAGE | Cupriavidus necator |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
rapid inactivation above | Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
phosphate buffer | Cupriavidus necator |
7.9 | - |
Tris-HCl buffer | Cupriavidus necator |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | completely inactive below pH 5.5 and above pH 10.0 | Cupriavidus necator |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
optimal stability in presence of 10 mM KNO3 | Cupriavidus necator |
8 | - |
rapid decrease of stability above | Cupriavidus necator |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | 0.9 mol of FMN per mol of enzyme | Cupriavidus necator | |
NAD+ | - |
Cupriavidus necator |